PuSH - Publication Server of Helmholtz Zentrum München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Weichmann, F.* ; Hett, R.* ; Schepers, A. ; Ito-Kureha, T.* ; Flatley, A. ; Slama, K.* ; Hastert, F.D.* ; Angstman, N.B.* ; Cardoso, M.C.* ; König, J.* ; Hüttelmaier, S.* ; Dieterich, C.* ; Canzar, S.* ; Helm, M.* ; Heissmeyer, V. ; Feederle, R. ; Meister, G.*

Validation strategies for antibodies targeting modified ribonucleotides.

RNA 26, 1489-1506 (2020)
Postprint DOI
Chemical modifications are found on almost all RNAs and affect their coding and noncoding functions. The identification of m(6)A on mRNA and its important role in gene regulation stimulated the field to investigate whether additional modifications are present on mRNAs. Indeed, modifications including m(1)A, m(5)C, m(7)G, 2'-OMe, and Psi were detected. However, since their abundances are low and tools used for their corroboration are often not well characterized, their physiological relevance remains largely elusive. Antibodies targeting modified nucleotides are often used but have limitations such as low affinity or specificity. Moreover, they are not always well characterized and due to the low abundance of the modification, particularly on mRNAs, generated data sets might resemble noise rather than specific modification patterns. Therefore, it is critical that the affinity and specificity is rigorously tested using complementary approaches. Here, we provide an experimental toolbox that allows for testing antibody performance prior to their use.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Scopus
Cited By
Altmetric
4.320
0.890
3
9
Tags
Annotations
Special Publikation
Hide on homepage

Edit extra information
Edit own tags
Private
Edit own annotation
Private
Hide on publication lists
on hompage
Mark as special
publikation
Publication type Article: Journal article
Document type Scientific Article
Keywords Monoclonal Antibodies ; Affinity ; M(6)a ; M(5)c ; Validation ; Base Modifications; Messenger-rna; Ribosomal-rna; M(6)a; Methylation; Pseudouridylation; Nuclear; N-6-methyladenosine; 5-methylcytosine; Proteins
Language english
Publication Year 2020
HGF-reported in Year 2020
ISSN (print) / ISBN 1355-8382
e-ISSN 1469-9001
Journal RNA
Quellenangaben Volume: 26, Issue: 10, Pages: 1489-1506 Article Number: , Supplement: ,
Publisher Cold Spring Harbor Laboratory Press
Publishing Place 1 Bungtown Rd, Cold Spring Harbor, Ny 11724 Usa
Reviewing status Peer reviewed
Institute(s) CF Monoclonal Antibodies (CF-MAB)
Research Unit Molecular Immune Regulation (AMIR)
POF-Topic(s) 30201 - Metabolic Health
30203 - Molecular Targets and Therapies
Research field(s) Helmholtz Diabetes Center
Immune Response and Infection
PSP Element(s) G-502210-001
G-501712-001
DOI 10.1261/rna.076026.120
WOS ID WOS:000570798100015
Scopus ID 85091191629
Erfassungsdatum 2020-11-06
[➜Report correction]