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Klena, N.* ; Le Guennec, M.* ; Tassin, A.M.* ; van den Hoek, H. ; Erdmann, P.S.* ; Schaffer, M.* ; Geimer, S.* ; Aeschlimann, G.* ; Kovacik, L.* ; Sadian, Y.* ; Goldie, K.N.* ; Stahlberg, H.* ; Engel, B.D. ; Hamel, V.* ; Guichard, P.*

Architecture of the centriole cartwheel-containing region revealed by cryo-electron tomography.

EMBO J. 39:e106246 (2020)

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	Open Access Gold (Paid Option)

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Centrioles are evolutionarily conserved barrels of microtubule triplets that form the core of the centrosome and the base of the cilium. While the crucial role of the proximal region in centriole biogenesis has been well documented, its native architecture and evolutionary conservation remain relatively unexplored. Here, using cryo-electron tomography of centrioles from four evolutionarily distant species, we report on the architectural diversity of the centriole's proximal cartwheel-bearing region. Our work reveals that the cartwheel central hub is constructed from a stack of paired rings with cartwheel inner densities inside. In bothParameciumandChlamydomonas, the repeating structural unit of the cartwheel has a periodicity of 25 nm and consists of three ring pairs, with 6 radial spokes emanating and merging into a single bundle that connects to the microtubule triplet via the D2-rod and the pinhead. Finally, we identified that the cartwheel is indirectly connected to the A-C linker through the triplet base structure extending from the pinhead. Together, our work provides unprecedented evolutionary insights into the architecture of the centriole proximal region, which underlies centriole biogenesis.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Cartwheel ; Centriole ; Cryo-electron Tomography ; Cryo-focused Ion Beam Milling ; In Situ; 9-fold Symmetry; Proteomic Analysis; Basal Bodies; Chlamydomonas; Protein; Sas-6; Duplication; Ultrastructure; Identification; Bld10/cep135

ISSN (print) / ISBN 0261-4189

e-ISSN 1460-2075

Journal EMBO Journal, The

Quellenangaben Volume: 39, Issue: 22, Article Number: e106246

Publisher Wiley

Publishing Place Heidelberg, Germany

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Helmholtz Pioneer Campus (HPC)

Grants Max Plank Society
Helmholtz Zentrum Munchen
European Research Council
Swiss National Science Foundation (SNSF)