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Quarles, C.D.* ; Macke, M.* ; Michalke, B. ; Zischka, H. ; Karst, U.* ; Sullivan, P.* ; Field, M.P.*

LC-ICP-MS method for the determination of "extractable copper" in serum.

Metallomics 12, 1348-1355 (2020)
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Copper is an essential element for biological functions within humans and animals. There are several known diseases associated with Cu deficiency or overload, such as Menkes disease and Wilson disease, respectively. A common clinical method for determining extractable Cu levels in serum, which is thought to be potentially dangerous if in excess, is to subtract the value of tightly incorporated Cu in ceruloplasmin from total serum Cu. In this work, an automated sample preparation and liquid chromatography (LC) system was combined with inductively coupled plasma-mass spectrometry (ICP-MS) to determine bound Cu and extractable Cu in serum. This LC-ICP-MS method took 250 s for sample preparation and analysis, followed by a column recondition/system reset, thus, a 6 minute sample-to-sample time including sample preparation. The method was validated using serum collected from either control (Atp7b(+/-)) or Wilson disease rats (Atp7b(-/-)). The extractable Cu was found to be 4.0 +/- 2.3 mu M Cu in healthy control rats, but 2.1 +/- 0.6 mu M Cu in healthy Wilson rats, and 27 +/- 16 mu M Cu in diseased Wilson rats, respectively. In addition, the extractable Cu/bound Cu ratio was found to be 6.4 +/- 3.5%, 38 +/- 29%, and 34 +/- 22%, respectively. These results suggest that the developed method could be of diagnostic value for Wilson disease, and possibly other copper related diseases.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Wilson Disease Gene; Exchange Chromatography; Alzheimers-disease; Menkes Disease; Speciation; Plasma; Ceruloplasmin; Iron; Cholesterol; Diagnosis
ISSN (print) / ISBN 1756-5901
e-ISSN 1756-591X
Journal Metallomics
Quellenangaben Volume: 12, Issue: 9, Pages: 1348-1355 Article Number: , Supplement: ,
Publisher Royal Society of Chemistry (RSC)
Publishing Place Thomas Graham House, Science Park, Milton Rd, Cambridge Cb4 0wf, Cambs, England
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Research Unit Analytical BioGeoChemistry (BGC)
Institute of Molecular Toxicology and Pharmacology (TOXI)