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Donau, C.* ; Späth, F.* ; Sosson, M.* ; Kriebisch, B.A.K.* ; Schnitter, F.* ; Tena-Solsona, M.* ; Kang, H.-S. ; Salibi, E.* ; Sattler, M. ; Mutschler, H.* ; Boekhoven, J.*

Active coacervate droplets as a model for membraneless organelles and protocells.

Nat. Commun. 11:5167 (2020)
Publ. Version/Full Text DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Membraneless organelles like stress granules are active liquid-liquid phase-separated droplets that are involved in many intracellular processes. Their active and dynamic behavior is often regulated by ATP-dependent reactions. However, how exactly membraneless organelles control their dynamic composition remains poorly understood. Herein, we present a model for membraneless organelles based on RNA-containing active coacervate droplets regulated by a fuel-driven reaction cycle. These droplets emerge when fuel is present, but decay without. Moreover, we find these droplets can transiently up-concentrate functional RNA which remains in its active folded state inside the droplets. Finally, we show that in their pathway towards decay, these droplets break apart in multiple droplet fragments. Emergence, decay, rapid exchange of building blocks, and functionality are all hallmarks of membrane-less organelles, and we believe that our work could be powerful as a model to study such organelles.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Phase-separation; P Granules; Rna; Complexes
Language english
Publication Year 2020
HGF-reported in Year 2020
ISSN (print) / ISBN 2041-1723
e-ISSN 2041-1723
Journal Nature Communications
Quellenangaben Volume: 11, Issue: 1, Pages: , Article Number: 5167 Supplement: ,
Publisher Nature Publishing Group
Publishing Place London
Reviewing status Peer reviewed
Institute(s) Institute of Structural Biology (STB)
POF-Topic(s) 30203 - Molecular Targets and Therapies
Research field(s) Enabling and Novel Technologies
PSP Element(s) G-503000-001
Grants Projekt DEAL
DOI 10.1038/s41467-020-18815-9
WOS ID WOS:000582680400003
Scopus ID 85092555843
PubMed ID 33056997
Erfassungsdatum 2020-11-24
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