PuSH - Publication Server of Helmholtz Zentrum München: Shortwave infrared polymethine fluorophores matched to excitation lasers enable non-invasive, multicolour in vivo imaging in real time.

Navigation

Home

Deutsch

Research

Advanced Search

Browse by ...

... Journal

... Publication Type

... Research Data

... Publication Year

Publication overview

Support & Contact

Contact persons

Help

Data protection

Cosco, E. ; Spearman, A.L.* ; Ramakrishnan, S. ; Lingg, J.G.P. ; Saccomano, M. ; Pengshung, M.* ; Arus, B.A. ; Wong, K.C.Y.* ; Glasl, S. ; Ntziachristos, V. ; Warmer, M. ; McLaughlin, R.R.* ; Bruns, O.T. ; Sletten, E.M.*

Shortwave infrared polymethine fluorophores matched to excitation lasers enable non-invasive, multicolour in vivo imaging in real time.

Nat. Chem. 12, 1123-1130 (2020)

Postprint

DOI

PMC

	Open Access Green

Abstract
Metrics
Extra information

High-resolution, multiplexed experiments are a staple in cellular imaging. Analogous experiments in animals are challenging, however, due to substantial scattering and autofluorescence in tissue at visible (350-700 nm) and near-infrared (700-1,000 nm) wavelengths. Here, we enable real-time, non-invasive multicolour imaging experiments in animals through the design of optical contrast agents for the shortwave infrared (SWIR, 1,000-2,000 nm) region and complementary advances in imaging technologies. We developed tunable, SWIR-emissive flavylium polymethine dyes and established relationships between structure and photophysical properties for this class of bright SWIR contrast agents. In parallel, we designed an imaging system with variable near-infrared/SWIR excitation and single-channel detection, facilitating video-rate multicolour SWIR imaging for optically guided surgery and imaging of awake and moving mice with multiplexed detection. Optimized dyes matched to 980 nm and 1,064 nm lasers, combined with the clinically approved indocyanine green, enabled real-time, three-colour imaging with high temporal and spatial resolutions.

Altmetric

Additional Metrics?

[➜Log in]

Edit extra informations Login

Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Fluorescence; Nanoparticles; Wavelengths; Emission; Dyes

ISSN (print) / ISBN 1755-4330

e-ISSN 1755-4349

Journal Nature chemistry

Quellenangaben Volume: 12, Issue: 12, Pages: 1123-1130

Publisher Nature Publishing Group

Publishing Place London

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Helmholtz Pioneer Campus (HPC)
Institute of Biological and Medical Imaging (IBMI)

Grants NIH
Helmholtz Zentrum Munchen
UCLA, Sloan Research Award
Emmy-Noether-Programme of DFG
NSF