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Dutta, R.* ; Mandal, S. ; Lin, H.-C. ; Raz, T.* ; Kind, A.* ; Schnieke, A.* ; Razansky, D.

Brilliant cresyl blue enhanced optoacoustic imaging enables non-destructive imaging of mammalian ovarian follicles for artificial reproduction.

J. R. Soc. Interface 17:20200776 (2020)

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In the field of reproductive biology, there is a strong need for a suitable tool capable of non-destructive evaluation of oocyte viability and function. We studied the application of brilliant cresyl blue (BCB) as an intra-vital exogenous contrast agent using multispectral optoacoustic tomography (MSOT) for visualization of porcine ovarian follicles. The technique provided excellent molecular sensitivity, enabling the selection of competent oocytes without disrupting the follicles. We further conducted in vitro embryo culture, molecular analysis (real-time and reverse transcriptase polymerase chain reaction) and DNA fragmentation analysis to comprehensively establish the safety of BCB-enhanced MSOT imaging in monitoring oocyte viability. Overall, the experimental results suggest that the method offers a significant advance in the use of contrast agents and molecular imaging for reproductive studies. Our technique improves the accurate prediction of ovarian reserve significantly and, once standardized for in vivo imaging, could provide an effective tool for clinical infertility management.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Artificial Reproductive Technology ; Brilliant Cresyl Blue ; Medical Imaging ; Optoacoustic Imaging ; Veterinary Medicine; Selection; Oocytes; Tomography; Apoptosis

ISSN (print) / ISBN 1742-5689

e-ISSN 1742-5662

Journal Interface : journal of the Royal Society

Quellenangaben Volume: 17, Issue: 172, Article Number: 20200776

Publisher Royal Society of London

Publishing Place London

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Biological and Medical Imaging (IBMI)

Grants Deutscher Akademischer Austauschdienst