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Burkhardt, R. ; Gora, T.* ; Fingerle, A.A.* ; Sauter, A.P.* ; Meurer, F.* ; Umkehrer, S.* ; Von Teuffenbach, M.* ; Kampfer, S.* ; Schilling, D. ; Feuchtinger, A. ; Walch, A.K. ; Rummeny, E.* ; Combs, S.E. ; Schmid, T.E. ; Pfeiffer, F.* ; Wilkens, J.J.* ; Herzen, J.*

Early detection of radiation-induced lung damage with X-ray dark-field radiography in mice.

Eur. Radiol. 31, 4175–4183 (2021)
Publ. Version/Full Text Research data DOI PMC
Open Access Gold (Paid Option)
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Objective: Assessing the advantage of x-ray dark-field contrast over x-ray transmission contrast in radiography for the detection of developing radiation-induced lung damage in mice. Methods: Two groups of female C57BL/6 mice (irradiated and control) were imaged obtaining both contrasts monthly for 28 weeks post irradiation. Six mice received 20 Gy of irradiation to the entire right lung sparing the left lung. The control group of six mice was not irradiated. A total of 88 radiographs of both contrasts were evaluated for both groups based on average values for two regions of interest, covering (irradiated) right lung and healthy left lung. The ratio of these average values, R, was distinguished between healthy and damaged lungs for both contrasts. The time-point when deviations of R from healthy lung exceeded 3σ was determined and compared among contrasts. The Wilcoxon-Mann-Whitney test was used to test against the null hypothesis that there is no difference between both groups. A selection of 32 radiographs was assessed by radiologists. Sensitivity and specificity were determined in order to compare the diagnostic potential of both contrasts. Inter-reader and intra-reader accuracy were rated with Cohen’s kappa. Results: Radiation-induced morphological changes of lung tissue caused deviations from the control group that were measured on average 10 weeks earlier with x-ray dark-field contrast than with x-ray transmission contrast. Sensitivity, specificity, and accuracy doubled using dark-field radiography. Conclusion: X-ray dark-field radiography detects morphological changes of lung tissue associated with radiation-induced damage earlier than transmission radiography in a pre-clinical mouse model. Key Points: • Significant deviations from healthy lung due to irradiation were measured after 16 weeks with x-ray dark-field radiography (p = 0.004). • Significant deviations occur on average 10 weeks earlier for x-ray dark-field radiography in comparison to x-ray transmission radiography. • Sensitivity and specificity doubled when using x-ray dark-field radiography instead of x-ray transmission radiography.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Lung ; Mice ; Radiography ; Sensitivity And Specificity ; X-rays; In-vivo Assessment; Computed-tomography; High-resolution; Ct; Irradiation; Emphysema; Diagnosis; Fibrosis; Model
ISSN (print) / ISBN 0938-7994
e-ISSN 1432-1084
Journal European Radiology
Quellenangaben Volume: 31, Issue: , Pages: 4175–4183 Article Number: , Supplement: ,
Publisher Springer
Publishing Place One New York Plaza, Suite 4600, New York, Ny, United States
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Radiation Medicine (IRM)
Research Unit Analytical Pathology (AAP)
Grants European Research Council
Deutsche Forschungsgemeinschaft
Karlsruhe Institute of Technology