PuSH - Publication Server of Helmholtz Zentrum München

Guggenberger, C. ; Ilgen, D. ; Adamski, J.

Functional analysis of cholesterol biosynthesis by RNA interference.

J. Steroid Biochem. Mol. Biol. 104, 105-109 (2007)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Inborn errors of cholesterol biosynthesis caused by dysfunctionality of single enzymes are known to cause severe malformation syndromes like X-linked chondrodysplasia punctata (CDPX2), CHILD syndrome or Smith-Lemli-Opitz-syndrome (SLOS). In this study we established the method of RNA interference (RNAi) for analyzing the molecular mechanisms underlying disrupted cholesterol biosynthesis. For different genes involved in the cholesterol biosynthesis pathway-NAD(P) dependent steroid dehydrogenase-like (NSDHL), 17-beta hydroxysteroid dehydrogenase type 7 (HSD17B7) and emopamil binding protein (EBP)-shRNA sequences were designed and tested for their effectiveness. For a better comparability of the experiments and to avoid different transfection efficiencies, examined shRNA sequences which reached a knock down of at least 80% were stably transfected in a HeLa cell line with a tetracycline-regulated expression (HeLa T-REx). These stable transfected cell lines represent novel tools for the analysis of cholesterol biosynthesis.
Altmetric
Additional Metrics?
[➜Log in]
Edit extra informations Login
Publication type Article: Journal article
Document type Scientific Article
Keywords Cholesterol biosynthesis; RNA interference; HSD17B7; NSDHL; EBP
ISSN (print) / ISBN 0960-0760
e-ISSN 0960-0760
Journal Journal of Steroid Biochemistry and Molecular Biology, The
Quellenangaben Volume: 104, Issue: 3-5, Pages: 105-109 Article Number: , Supplement: ,
Publisher Elsevier
Reviewing status Peer reviewed
Institute(s) Molekulare Endokrinologie und Metabolismus (MEM)