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A biomimetic, copolymeric membrane for cell-stretch experiments with pulmonary epithelial cells at the air-liquid interface.
Adv. Func. Mat., DOI: 10.1002/adfm.202004707 (2020)
Chronic respiratory diseases are among the leading causes of death worldwide, but only symptomatic therapies are available for terminal illness. This in part reflects a lack of biomimetic in vitro models that can imitate the complex environment and physiology of the lung. Here, a copolymeric membrane consisting of poly(epsilon-)caprolactone and gelatin with tunable properties, resembling the main characteristics of the alveolar basement membrane is introduced. The thin bioinspired membrane (0.5 mu m) is stretchable (up to 25% linear strain) with appropriate surface wettability and porosity for culturing lung epithelial cells under air-liquid interface conditions. The unique biphasic concept of this membrane provides optimum characteristics for initial cell growth (phase I) and then switch to biomimetic properties for cyclic cell-stretch experiments (phase II). It is showed that physiologic cyclic mechanical stretch improves formation of F-actin cytoskeleton filaments and tight junctions while non-physiologic over-stretch induces cell apoptosis, activates inflammatory response (IL-8), and impairs epithelial barrier integrity. It is also demonstrated that cyclic physiologic stretch can enhance the cellular uptake of nanoparticles. Since this membrane offers considerable advantages over currently used membranes, it may lead the way to more biomimetic in vitro models of the lung for translation of in vitro response studies into clinical outcome.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Alveolar‐ ; Capillary Barrier ; Cyclic Mechanical Stretch ; Hybrid Polymers ; In Vitro Cell‐ ; Stretch Model ; Tunable Ultra‐ ; Thin Biphasic Membrane; Tight Junction; Nanoparticles; Permeability; Absorption; Delivery; Scaffold; Science; Barrier; Blood
Language
english
Publication Year
2020
HGF-reported in Year
2020
ISSN (print) / ISBN
1616-301X
e-ISSN
1616-3028
Journal
Advanced functional materials
Publisher
Wiley
Publishing Place
Weinheim
Reviewing status
Peer reviewed
Institute(s)
Institute of Lung Health and Immunity (LHI)
POF-Topic(s)
30202 - Environmental Health
Research field(s)
Lung Research
PSP Element(s)
G-505000-008
Grants
Projekt DEAL
WOS ID
WOS:000597415000001
Erfassungsdatum
2021-01-12