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Schinke, H.* ; Heider, T. ; Herkommer, T. ; Simon, F.* ; Blancke Soares, A.* ; Kranz, G.* ; Samaga, D. ; Dajka, L. ; Feuchtinger, A. ; Walch, A.K. ; Valeanu, L.* ; Walz, C.* ; Kirchner, T.* ; Canis, M.* ; Baumeister, P. ; Belka, C. ; Maihöfer, C. ; Marschner, S. ; Pflugradt, U. ; Ganswindt, U.* ; Hess J. ; Zitzelsberger, H. ; Gires, O.

Digital scoring of EpCAM and slug expression as prognostic markers in head and neck squamous cell carcinomas.

Mol. Oncol. 15, 1040-1053 (2021)
Publ. Version/Full Text DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Head and neck squamous cell carcinomas (HNSCCs) have poor clinical outcome owing to therapy resistance and frequent recurrences that are among others attributable to tumor cells in partial epithelial-to-mesenchymal transition (pEMT). We compared side-by-side software-based and visual quantification of immunohistochemistry (IHC) staining of epithelial marker EpCAM and EMT regulator Slug in n = 102 primary HNSCC to assess optimal analysis protocols. IHC scores incorporated expression levels and percentages of positive cells. Digital and visual evaluation of membrane-associated EpCAM yielded correlating scorings, whereas visual evaluation of nuclear Slug resulted in significantly higher overall scores. Multivariable Cox proportional hazard analysis defined the median EpCAM expression levels resulting from visual quantification as an independent prognostic factor of overall survival. Slug expression levels resulting from digital quantification were an independent prognostic factor of recurrence-free survival, locoregional recurrence-free survival, and disease-specific survival. Hence, we propose to use visual assessment for the membrane-associated EpCAM protein, whereas nuclear protein Slug assessment was more accurate following digital measurement.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Antigen Scoring ; Emt ; Epcam ; Hnscc ; Slug; Survival; Snail
ISSN (print) / ISBN 1574-7891
e-ISSN 1878-0261
Journal Molecular Oncology
Quellenangaben Volume: 15, Issue: 4, Pages: 1040-1053 Article Number: , Supplement: ,
Publisher Elsevier
Publishing Place Amsterdam [u.a.]
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) CCG Personalized Radiotherapy in Head and Neck Cancer (KKG-KRT)
Translational Metabolic Oncology (TMO)
Research Unit Analytical Pathology (AAP)
Grants Deutsche Forschungsgemeinschaft

Bayerisches Staatsministerium für Wirtschaft, Energie und Technologie BayBIO