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Siehler, J. ; Blöchinger, A. ; Akgün, M. ; Wang, X. ; Shahryari, A. ; Geerlof, A. ; Lickert, H. ; Burtscher, I.

Generation of a heterozygous C-peptide-mCherry reporter human iPSC line (HMGUi001-A-8).

Stem Cell Res. 50:102126 (2021)
Publ. Version/Full Text DOI PMC
Open Access Gold
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The peptide hormone insulin produced by pancreatic β-cells undergoes post-transcriptional processing before secretion. In particular, C-peptide is cleaved from pro-insulin to generate mature insulin. Here, we introduce a C-peptide-mCherry human iPSC line (HMGUi001-A-8). The line was generated by CRISPR/Cas9 mediated heterozygous insertion of the mCherry sequence into exon 3 of the insulin locus. We demonstrate that the line is pluripotent and efficiently differentiates towards pancreatic β-like cells, which localize a red fluorescent C-peptide-mCherry fusion protein in insulin containing granules. Hence, the HMGUi001-A-8 line is a valuable resource to purify derived β-like cells and follow insulin-containing granules in real time.
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Publication type Article: Journal article
Document type Scientific Article
Language english
Publication Year 2021
Prepublished in Year 2020
HGF-reported in Year 2020
ISSN (print) / ISBN 1873-5061
e-ISSN 1876-7753
Journal Stem Cell Research
Quellenangaben Volume: 50, Issue: , Pages: , Article Number: 102126 Supplement: ,
Publisher Elsevier
Publishing Place Radarweg 29, 1043 Nx Amsterdam, Netherlands
Reviewing status Peer reviewed
Institute(s) Institute of Diabetes and Regeneration Research (IDR)
Institute of Structural Biology (STB)
Institute of Stem Cell Research (ISF)
POF-Topic(s) 30201 - Metabolic Health
90000 - German Center for Diabetes Research
30203 - Molecular Targets and Therapies
30204 - Cell Programming and Repair
Research field(s) Helmholtz Diabetes Center
Enabling and Novel Technologies
Stem Cell and Neuroscience
PSP Element(s) G-502300-001
G-501900-231
G-503000-001
G-500800-001
Grants HumEN consortium - European Union's Seventh Framework Programme for Research, Technological Development and Demonstration
Deutsches Zentrum fur Diabetesforschung (DZD)
Helmholtz-Gemeinschaft (Helmholtz Portfolio Theme 'Metabolic Dysfunction and Common Disease)
DOI 10.1016/j.scr.2020.102126
WOS ID WOS:000614260000018
Scopus ID 85098194942
PubMed ID 33373890
Erfassungsdatum 2021-01-03
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