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Wahman, R.* ; Sauvetre, A. ; Schröder, P. ; Moser, S.* ; Letzel, T.*

Untargeted metabolomics studies on drug-incubated phragmites Australis profiles.

Metabolites 11:2 (2021)
Publ. Version/Full Text Research data DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Plants produce a huge number of functionally and chemically different natural products that play an important role in linking the plant with the adjacent environment. Plants can also absorb and transform external organic compounds (xenobiotics). Currently there are only a few studies concerning the effects of xenobiotics and their transformation products on plant metabolites using a mass spectrometric untargeted screening strategy. This study was designed to investigate the changes of the Phragmites australis metabolome following/after diclofenac or carbamazepine incubation, using a serial coupling of reversed-phase liquid chromatography (RPLC) and hydrophilic interaction liquid chromatography (HILIC) combined with accurate high-resolution time-of-flight mass spectrometer (TOF-MS). An untargeted screening strategy of metabolic fingerprints was developed to purposefully compare samples from differently treated P. australis plants, revealing that P. australis responded to each drug differently. When solvents with significantly different polarities were used, the metabolic profiles of P. australis were found to change significantly. For instance, the production of polyphenols (such as quercetin) in the plant increased after diclofenac incubation. Moreover, the pathway of unsaturated organic acids became more prominent, eventually as a reaction to protect the cells against reactive oxygen species (ROS). Hence, P. australis exhibited an adaptive mechanism to cope with each drug. Consequently, the untargeted screening approach is essential for understanding the complex response of plants to xenobiotics.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Carbamazepine ; Diclofenac ; Orthogonal Partial Least Square-discriminant Analysis (opls-da) ; P. Australis Metabolic Profile ; Untargeted Metabolomics
Language english
Publication Year 2021
Prepublished in Year 2020
HGF-reported in Year 2020
ISSN (print) / ISBN 2218-1989
e-ISSN 2218-1989
Journal Metabolites
Quellenangaben Volume: 11, Issue: 1, Pages: , Article Number: 2 Supplement: ,
Publisher MDPI
Publishing Place St Alban-anlage 66, Ch-4052 Basel, Switzerland
Reviewing status Peer reviewed
Institute(s) Research Unit Comparative Microbiome Analysis (COMI)
POF-Topic(s) 30202 - Environmental Health
Research field(s) Environmental Sciences
PSP Element(s) G-504700-003
Grants Cultural Affairs and Mission Sector of the Egyptian Ministry of Higher Education
Bavarian State Ministry of the Environment and Consumer Protection
DOI 10.3390/metabo11010002
WOS ID WOS:000610388600001
Scopus ID 85098539299
PubMed ID 33375173
Erfassungsdatum 2021-02-22
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