PuSH - Publication Server of Helmholtz Zentrum München: Specific induction of double negative B cells during protective and pathogenic immune responses.

Navigation

Home

Deutsch

Research

Advanced Search

Browse by ...

... Journal

... Publication Type

... Research Data

... Publication Year

Publication overview

Support & Contact

Contact persons

Help

Data protection

Ruschil, C.* ; Gabernet, G.* ; Lepennetier, G.* ; Heumos, S.* ; Kaminski, M.* ; Hracsko, Z.* ; Irmler, M. ; Beckers, J. ; Ziemann, U.* ; Nahnsen, S.* ; Owens, G.P.* ; Bennett, J.L.* ; Hemmer, B.* ; Kowarik, M.C.*

Specific induction of double negative B cells during protective and pathogenic immune responses.

Front. Immunol. 11:606338 (2020)

Publ. Version/Full Text

Research data

DOI

PMC

	Open Access Gold

Abstract
Metrics
Extra information

Double negative (DN) (CD19+CD20lowCD27-IgD-) B cells are expanded in patients with autoimmune and infectious diseases; however their role in the humoral immune response remains unclear. Using systematic flow cytometric analyses of peripheral blood B cell subsets, we observed an inflated DN B cell population in patients with variety of active inflammatory conditions: myasthenia gravis, Guillain-Barré syndrome, neuromyelitis optica spectrum disorder, meningitis/encephalitis, and rheumatic disorders. Furthermore, we were able to induce DN B cells in healthy subjects following vaccination against influenza and tick borne encephalitis virus. Transcriptome analysis revealed a gene expression profile in DN B cells that clustered with naïve B cells, memory B cells, and plasmablasts. Immunoglobulin VH transcriptome sequencing and analysis of recombinant antibodies revealed clonal expansion of DN B cells that were targeted against the vaccine antigen. Our study suggests that DN B cells are expanded in multiple inflammatory neurologic diseases and represent an inducible B cell population that responds to antigenic stimulation, possibly through an extra-follicular maturation pathway.

Altmetric

Additional Metrics?

[➜Log in]

Edit extra informations Login

Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Autoimmune Disorders ; B Cells ; Double Negative B Cells ; Influenza Vaccination ; Neuromyelitis Optica Spectrum Disorder ; Tbe Vaccination ; Vaccination; Population; Expression; Antibody; Cd27; Infection; Prominent; Subsets; Toolkit

ISSN (print) / ISBN 1664-3224

e-ISSN 1664-3224

Journal Frontiers in Immunology

Quellenangaben Volume: 11, Article Number: 606338

Publisher Frontiers

Publishing Place Avenue Du Tribunal Federal 34, Lausanne, Ch-1015, Switzerland

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Experimental Genetics (IEG)

Grants NIHR01EY022936
German Ministry of Research and Education (BMBF)
Ministry of Science, Research and Arts of Baden-Wurttemberg (MWK) for the Science Data Centre project BioDATEN
Central Innovation Program (ZIM) for SMEs of the Federal Ministry for Economic Affairs and Energy of Germany
Federal Ministry of Education and Research (BMBF)
Baden-Wurttemberg Ministry of Science as part of the Excellence Strategy of the German Federal and State Governments
DFG
Helmholtz Alliance 'Aging and Metabolic Programming, AMPro'
MultipleMS EU consortium
German Federal Ministry for Education and Research [Munich]
Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany's Excellence Strategy
DIFUTURE (Data Integration for Future Medicine)
Munich Cluster for Systems Neurology (SyNergy)
fortune/PATE from the medical faculty, Eberhard-Karls University of Tubingen