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Duffner, C. ; Holzapfel, S.* ; Wunderlich, A.* ; Einsiedl, F.* ; Schloter, M. ; Schulz, S.

Dechloromonas and close relatives prevail hydrogenotrophic denitrification in stimulated microcosms with oxic aquifer material.

FEMS Microbiol. Ecol. 97:fiab004 (2021)
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Globally occurring nitrate pollution in groundwater is harming the environment and human health. In situ hydrogen addition to stimulate denitrification has been proposed as a remediation strategy. However, observed nitrite accumulation and incomplete denitrification are severe drawbacks that possibly stem from the specific microbial community composition. We set up a microcosm experiment comprising sediment and groundwater from a nitrate polluted oxic oligotrophic aquifer. After the microcosms were sparged with hydrogen gas, samples were taken regularly within 122 h for nitrate and nitrite measurements, community composition analysis via 16S rRNA gene amplicon sequencing, and gene and transcript quantification via qPCR of reductase genes essential for complete denitrification. The highest nitrate reduction rates and greatest increase in bacterial abundance coincided with the 15.3-fold increase in Rhodocyclaceae, specifically six ASVs that are closely related to the genus Dechloromonas. The denitrification reductase genes napA, nirS, and clade I nosZ also increased significantly over the observation period. We conclude that taxa of the genus Dechloromonas are the prevailing hydrogenotrophic denitrifiers in this nitrate polluted aquifer and the ability of hydrogenotrophic denitrification under the given conditions is species-specific.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Rhodocyclaceae ; Denitrification Genes ; Groundwater ; Hydrogen Oxidation ; Nitrate Pollution ; Remediation; Denitrifying Bacteria; Nitrate; Groundwater; Water; Perchlorate; Diversity; Patterns; Kinetics; Biofilm; Reveal
ISSN (print) / ISBN 0168-6496
e-ISSN 1574-6941
Journal FEMS Microbiology Ecology
Quellenangaben Volume: 97, Issue: 3, Pages: , Article Number: fiab004 Supplement: ,
Publisher Wiley
Publishing Place Oxford
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Research Unit Comparative Microbiome Analysis (COMI)
Grants TUM International Graduate School of Science and Engineering(IGSSE)