Home
Deutsch
Advanced Search
Browse by ...
Publication overview
Contact persons
Help
DOI
PMC
 |
|
Open Access Green as soon as Postprint is submitted to ZB.
Isolation and purification of mitochondria from cell culture for proteomic analyses.
In: Proteomic Profiling. Berlin [u.a.]: Springer, 2021. 411-419 (Methods Mol. Biol. ; 2261)
In-depth analysis of the mitochondrial proteome can be greatly improved by analyzing isolated mitochondria instead of whole cells. However, isolation of sufficient amounts of mitochondria from cell culture has proven to be notoriously difficult due to small sample size. Thus, we have developed a reproducible, controllable, and highly customizable method to isolate high microgram to low milligram amounts of intact mitochondria from cell culture samples along with an optional density gradient purification. This chapter provides a methodological update of our approach and underlines the excellent quality and coverage of the mitochondrial proteome of crude and purified mitochondria from cultured liver cancer cell lines.
Altmetric
Additional Metrics?
Edit extra informations
Login
Publication type
Article: Edited volume or book chapter
Keywords
Balch Homogenizer ; Cell Culture ; Mitochondria ; Proteomics
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Book Volume Title
Proteomic Profiling
Journal
Methods in Molecular Biology
Quellenangaben
Volume: 2261,
Pages: 411-419
Publisher
Springer
Publishing Place
Berlin [u.a.]
Non-patent literature
Publications
Reviewing status
Peer reviewed