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Microfluidics for single-cell lineage tracking over time to characterize transmission of phenotypes in Saccharomyces cerevisiae.
STAR Protoc. 1:100228 (2020)
The budding yeast Saccharomyces cerevisiae is an excellent model organism to dissect the maintenance and inheritance of phenotypes due to its asymmetric division. This requires following individual cells over time as they go through divisions to define pedigrees. Here, we provide a detailed protocol for collecting and analyzing time-lapse imaging data of yeast cells. The microfluidics protocol can achieve improved time resolution for single-cell tracking to enable characterization of maintenance and inheritance of phenotypes. For complete details on the use and execution of this protocol, please refer to Bheda et al. (2020a).
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Cell Biology ; Microscopy ; Model Organisms ; Single Cell
Language
english
Publication Year
2020
HGF-reported in Year
2020
e-ISSN
2666-1667
Journal
STAR Protocols
Quellenangaben
Volume: 1,
Issue: 3,
Article Number: 100228
Reviewing status
Peer reviewed
Institute(s)
Institute of Functional Epigenetics (IFE)
POF-Topic(s)
30203 - Molecular Targets and Therapies
Research field(s)
Helmholtz Diabetes Center
PSP Element(s)
G-502800-001
Scopus ID
85122811892
PubMed ID
33377118
Erfassungsdatum
2021-02-09