PuSH - Publication Server of Helmholtz Zentrum München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Jung, S. ; Jakobs, K.F.K. ; Shein, M.* ; Schütz, A.K.* ; Mohr, F.* ; Stadler, H.* ; Stadler, D. ; Lucko, A. ; Altstetter, S. ; Wilsch, F. ; Deng, L. ; Protzer, U.

Efficient and reproducible depletion of hepatitis B virus from plasma derived extracellular vesicles.

J. Extra. Vesicles 10:e12040 (2020)
Publ. Version/Full Text DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Extracellular vesicles (EVs) are emerging fundamental players in viral infections by shuttling viral components, mediating immune responses and likely the spread of the virus. However, the obstacles involved in purifying EVs and removing contaminating viral particles in a reliable and effective manner bottlenecks the full potential for the development of clinical and diagnostic treatment options targeting EV. Because of the similarities in size, density, membrane composition and mode of biogenesis of EVs and virions there are no standardized approaches for virus-removal from EV preparations yet. Functional EV studies also require EV samples that are devoid of antibody contaminants. Consequently, the study of EVs in virology needs reliable and effective protocols to purify EVs and remove contaminating antibodies and viral particles. Here, we established a protocol for EV purification from hepatitis B virus (HBV)-containing plasma by a combination of size-exclusion chromatography and affinity-based purification. After purification, EV samples were free of virus-sized particles, HBV surface antigen, HBV core antigen, antibodies or infectious material. Viral genomic contamination was also decreased following purification. By using appropriate antibodies and size parameters, this protocol could potentially be applied to purification of EVs from other viral samples. In summary, we established a fast, reproducible and robust approach for the removal of HBV from EV preparations. Looking forward to the point of purifying EVs from clinical samples, this method should enable studies shedding light on the underlying mechanisms of EVs in viral infections and their diagnostic and prognostic potential.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Scopus
Cited By
Altmetric
14.976
2.212
3
5
Tags
Annotations
Special Publikation
Hide on homepage

Edit extra information
Edit own tags
Private
Edit own annotation
Private
Hide on publication lists
on hompage
Mark as special
publikation
Publication type Article: Journal article
Document type Scientific Article
Keywords Ev Purification ; Affinity‐based Purification ; Extracellular Vesicles ; Hepatitis B Virus ; Size‐exclusion Chromatography ; Virus Removal
Language english
Publication Year 2020
HGF-reported in Year 2020
ISSN (print) / ISBN 2001-3078
e-ISSN 2001-3078
Journal Journal of extracellular vesicles
Quellenangaben Volume: 10, Issue: 2, Pages: , Article Number: e12040 Supplement: ,
Publisher Taylor & Francis
Publishing Place [S.l.]
Reviewing status Peer reviewed
Institute(s) Institute of Virology (VIRO)
POF-Topic(s) 30203 - Molecular Targets and Therapies
Research field(s) Immune Response and Infection
PSP Element(s) G-502700-003
G-554300-001
Grants German Research Association (DFG)
DOI 10.1002/jev2.12040
WOS ID WOS:000610094700006
Scopus ID 85100169666
PubMed ID 33363711
Erfassungsdatum 2021-01-14
[➜Report correction]