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Acevedo, N.* ; Scala, G.* ; Merid, S.K.* ; Frumento, P.* ; Bruhn, S.* ; Andersson, A.* ; Ogris, C. ; Bottai, M.* ; Pershagen, G.* ; Koppelman, G.H.* ; Melén, E.* ; Sonnhammer, E.* ; Alm, J.* ; Söderhäll, C.* ; Kere, J.* ; Greco, D.* ; Scheynius, A.*

DNA methylation levels in mononuclear leukocytes from the mother and her child are associated with ige sensitization to allergens in early life.

Int. J. Mol. Sci. 22:801 (2021)
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DNA methylation changes may predispose becoming IgE-sensitized to allergens. We analyzed whether DNA methylation in peripheral blood mononuclear cells (PBMC) is associated with IgE sensitization at 5 years of age (5Y). DNA methylation was measured in 288 PBMC samples from 74 mother/child pairs from the birth cohort ALADDIN (Assessment of Lifestyle and Allergic Disease During INfancy) using the HumanMethylation450BeadChip (Illumina). PBMCs were obtained from the mothers during pregnancy and from their children in cord blood, at 2 years and 5Y. DNA methylation levels at each time point were compared between children with and without IgE sensitization to allergens at 5Y. For replication, CpG sites associated with IgE sensitization in ALADDIN were evaluated in whole blood DNA of 256 children, 4 years old, from the BAMSE (Swedish abbreviation for Children, Allergy, Milieu, Stockholm, Epidemiology) cohort. We found 34 differentially methylated regions (DMRs) associated with IgE sensitization to airborne allergens and 38 DMRs associated with sensitization to food allergens in children at 5Y (Sidak p ≤ 0.05). Genes associated with airborne sensitization were enriched in the pathway of endocytosis, while genes associated with food sensitization were enriched in focal adhesion, the bacterial invasion of epithelial cells, and leukocyte migration. Furthermore, 25 DMRs in maternal PBMCs were associated with IgE sensitization to airborne allergens in their children at 5Y, which were functionally annotated to the mTOR (mammalian Target of Rapamycin) signaling pathway. This study supports that DNA methylation is associated with IgE sensitization early in life and revealed new candidate genes for atopy. Moreover, our study provides evidence that maternal DNA methylation levels are associated with IgE sensitization in the child supporting early in utero effects on atopy predisposition.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Alladin ; Allergens ; Atopy ; Bamse ; Dna Methylation ; Epigenetics ; Ige Sensitization ; Maternal Effects
Language english
Publication Year 2021
HGF-reported in Year 2021
ISSN (print) / ISBN 1661-6596
e-ISSN 1422-0067
Journal International Journal of Molecular Sciences
Quellenangaben Volume: 22, Issue: 2, Pages: , Article Number: 801 Supplement: ,
Publisher MDPI
Publishing Place Basel
Reviewing status Peer reviewed
Institute(s) Institute of Computational Biology (ICB)
POF-Topic(s) 30205 - Bioengineering and Digital Health
Research field(s) Enabling and Novel Technologies
PSP Element(s) G-503800-001
Grants the Konung Gustaf V:s 80-årsfond
Swedish Foundation for Strategic Research
DOI 10.3390/ijms22020801
WOS ID WOS:000611315500001
Scopus ID 85099395844
PubMed ID 33466918
Erfassungsdatum 2021-03-26
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