PuSH - Publication Server of Helmholtz Zentrum München: Adult neural stem cell activation in mice is regulated by the day/night cycle and intracellular calcium dynamics.

Navigation

Home

Deutsch

Research

Advanced Search

Browse by ...

... Journal

... Publication Type

... Research Data

... Publication Year

Publication overview

Support & Contact

Contact persons

Help

Data protection

Gengatharan, A.* ; Malvaut, S.* ; Marymonchyk, A.* ; Ghareghani, M.* ; Snapyan, M.* ; Fischer-Sternjak, J. ; Ninkovic, J. ; Götz, M. ; Saghatelyan, A.*

Adult neural stem cell activation in mice is regulated by the day/night cycle and intracellular calcium dynamics.

Cell 184, 709-722.e13 (2021)

Publ. Version/Full Text

Postprint

Research data

DOI

PMC

	Open Access Green

Abstract
Metrics
Extra information

Neural stem cells (NSCs) in the adult brain transit from the quiescent state to proliferation to produce new neurons. The mechanisms regulating this transition in freely behaving animals are, however, poorly understood. We customized in vivo imaging protocols to follow NSCs for several days up to months, observing their activation kinetics in freely behaving mice. Strikingly, NSC division is more frequent during daylight and is inhibited by darkness-induced melatonin signaling. The inhibition of melatonin receptors affected intracellular Ca²⁺ dynamics and promoted NSC activation. We further discovered a Ca²⁺ signature of quiescent versus activated NSCs and showed that several microenvironmental signals converge on intracellular Ca²⁺ pathways to regulate NSC quiescence and activation. In vivo NSC-specific optogenetic modulation of Ca²⁺ fluxes to mimic quiescent-state-like Ca²⁺ dynamics in freely behaving mice blocked NSC activation and maintained their quiescence, pointing to the regulatory mechanisms mediating NSC activation in freely behaving animals.

Altmetric

Additional Metrics?

[➜Log in]

Edit extra informations Login

Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Crispr-cas9 ; Ca(2+) Signaling ; G-proteins ; Nscs ; Circadian Rhythm ; In vivo Imaging ; Melatonin ; Mini-endoscopes ; Neural Stem Cells ; Subventricular Zone

ISSN (print) / ISBN 0092-8674

e-ISSN 1097-4172

Journal Cell

Quellenangaben Volume: 184, Issue: 3, Pages: 709-722.e13

Publisher Cell Press

Publishing Place Cambridge, Mass.

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Stem Cell Research (ISF)

Grants Canadian Institute of Health Research (CIHR)
Roger de Spoelberch Foundation