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Lalaouna, D.* ; Fochesato, S.* ; Harir, M. ; Ortet, P.* ; Schmitt-Kopplin, P. ; Heulin, T.* ; Achouak, W.*

Amplifying and fine-tuning Rsm sRNAs expression and stability to optimize the survival of Pseudomonas brassicacerum in nutrient-poor environments.

Microorganisms 9:250 (2021)

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In the beneficial plant root-associated Pseudomonas brassicacearum strain NFM421, the GacS/GacA two-component system positively controls biofilm formation and the production of secondary metabolites through the synthesis of rsmX, rsmY and rsmZ. Here, we evidenced the genetic amplification of Rsm sRNAs by the discovery of a novel 110-nt long sRNA encoding gene, rsmX-2, generated by the duplication of rsmX-1 (formerly rsmX). Like the others rsm genes, its overexpression overrides the gacA mutation. We explored the expression and the stability of rsmX-1, rsmX-2, rsmY and rsmZ encoding genes under rich or nutrient-poor conditions, and showed that their amount is fine-tuned at the transcriptional and more interestingly at the post-transcriptional level. Unlike rsmY and rsmZ, we noticed that the expression of rsmX-1 and rsmX-2 genes was exclusively GacAdependent. The highest expression level and longest half-life for each sRNA were correlated with the highest ppGpp and cyclic-di-GMP levels and were recorded under nutrient-poor conditions. Together, these data support the view that the Rsm system in P. brassicacearum is likely linked to the stringent response, and seems to be required for bacterial adaptation to nutritional stress.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Gaca-dependent ; Nutritional Stress ; Pseudomonas Brassicacearum ; Rsm Srnas ; Srnas Stability

ISSN (print) / ISBN 2076-2607

e-ISSN 2076-2607

Journal Microorganisms

Quellenangaben Volume: 9, Issue: 2, Article Number: 250

Publisher MDPI

Publishing Place Basel

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Research Unit Analytical BioGeoChemistry (BGC)

Grants IRTELIS PhD program Grant from CEA