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Zissler, U.M. ; Jakwerth, C.A. ; Guerth, F. ; Lewitan, L. ; Rothkirch, S. ; Davidovic, M. ; Ulrich, M. ; Oelsner, M. ; Garn, H.* ; Schmidt-Weber, C.B. ; Chaker, A.

Allergen-specific immunotherapy induces the suppressive secretoglobin 1A1 in cells of the lower airways.

Allergy 76, 2461-2474 (2021)
Publ. Version/Full Text DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
BACKGROUND: While several systemic immunomodulatory effects of allergen-specific immunotherapy (AIT) have been discovered, local anti-inflammatory mechanisms in the respiratory tract are largely unknown. We sought to elucidate local and epithelial mechanisms underlying allergen-specific immunotherapy in a genome-wideapproach. METHODS: We induced sputum in hayfever patients and healthy controls during the pollen peak season and stratified patients by effective allergen-immunotherapy or as untreated. Sputum was directly processed after induction and subjected to whole transcriptome RNA microarray analysis. Nasal secretions were analyzed for Secretoglobin1A1(SCGB1A1) and IL-24 protein levels in an additional validation cohort at three defined time points during the three-year course of AIT. Subsequently, RNA was extracted and subjected to an array-based whole transcriptome analysis. RESULTS: AIT inhibited pro-inflammatory CXCL8, IL24 and CCL26mRNA expression, while SCGB1A1, IL7, CCL5, CCL23 and WNT5BmRNAs were induced independently of the asthma status and allergen season. In our validation cohort, local increase of SCGB1A1 occurred concomitantly with the reduction of local IL-24 in upper airways during the course of AIT. Additionally, SCGB1A1 was identified as a suppressor of epithelial gene expression. CONCLUSIONS: AIT induces a yet unknown local gene expression footprint in the lower airwaysthat on one hand appears to be a result of multiple regulatory pathways and on the other hand reveals SCGB1A1 as novel anti-inflammatory mediator of long-term allergen specific therapeutic intervention in the local environment.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Allergen-specific Immunotherapy ; Allergic Rhinitis ; Asthma ; Induced Sputum,tolerance
ISSN (print) / ISBN 0105-4538
e-ISSN 1398-9995
Journal Allergy
Quellenangaben Volume: 76, Issue: 8, Pages: 2461-2474 Article Number: , Supplement: ,
Publisher Wiley
Publishing Place 111 River St, Hoboken 07030-5774, Nj Usa
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute for Allergy Research (IAF)
Grants German Research Foundation (DFG)
Helmholtz Inflammation&Immunology (II)
German Center for Lung Research (DZL)