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Hansen, J. ; von Melchner, H.* ; Wurst, W.

Mutant non-coding RNA resource in mouse embryonic stem cells.

Dis. Model. Mech. 14:dmm047803 (2021)
Publ. Version/Full Text Research data DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Gene trapping is a high-throughput approach that has been used to introduce insertional mutations into the genome of mouse embryonic stem (ES) cells. It is performed with generic gene trap vectors that simultaneously mutate and report the expression of the endogenous gene at the site of insertion and provide a DNA sequence tag for the rapid identification of the disrupted gene. Large-scale international efforts assembled a gene trap library of 566,554 ES cell lines with single gene trap integrations distributed throughout the genome. Here, we re-investigated this unique library and identified mutations in 2202 non-coding RNA (ncRNA) genes, in addition to mutations in 12,078 distinct protein-coding genes. Moreover, we found certain types of gene trap vectors preferentially integrating into genes expressing specific long non-coding RNA (lncRNA) biotypes. Together with all other gene-trapped ES cell lines, lncRNA gene-trapped ES cell lines are readily available for functional in vitro and in vivo studies.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Gene Trap ; Long Non-coding Rna ; Mouse ; Mus Musculus ; Mutagenesis ; Mutation; Gene-trap Screen; Functional-analysis; Large-scale; Insertional Mutations; Multipurpose Alleles; Disruption; Mutagenesis; Expression; Pluripotency; Efficient
ISSN (print) / ISBN 1754-8403
e-ISSN 1754-8411
Journal Disease Models and Mechanisms
Quellenangaben Volume: 14, Issue: 2, Pages: , Article Number: dmm047803 Supplement: ,
Publisher Company of Biologists
Publishing Place Bidder Building, Station Rd, Histon, Cambridge Cb24 9lf, England
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Developmental Genetics (IDG)
Institute of Metabolism and Cell Death (MCD)
Grants project 'I-DCC: The International Data Coordination Centre' by European Commission
project 'EUCOMM' by European Commission
National Genome Research Network Plus Project 'From Disease Genes to Protein Pathways' by Bundesministerium fur Bildung, Wissenschaft und Forschung