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Stauffer, E. ; Weber, P. ; Heider, T. ; Dalke, C. ; Blutke, A. ; Walch, A.K. ; Burgstaller, G. ; Brix, N.* ; Lauber, K.* ; Zitzelsberger, H. ; Unger, K. ; Selmansberger, M.

Transcriptomic landscape of radiation-induced murine thyroid proliferative lesions.

Endocr. Relat. Cancer 28, 213-224 (2021)
Publ. Version/Full Text Postprint DOI PMC
Open Access Green
Thyroid carcinoma incidence rates in western societies are among the fastest rising, compared to all malignant tumors over the past two decades. While risk factors such as age and exposure to ionizing radiation are known, early-state carcinogenic processes or pre-lesions are poorly understood or unknown. This study aims at the identification and characterization of early-state radiation-associated neoplastic processes by histologic and transcriptomic analyses of thyroid tissues derived from a mouse model. Comprehensive histological examination of 246 thyroids (164 exposed, 82 non-exposed) was carried out. Proliferative and normal tissues from exposed cases and normal tissue from non-exposed cases were collected by laser-capture microdissection, followed by RNAseq transcriptomic profiling using a low input 3`-library preparation protocol, differential gene expression analysis and functional association by Gene Set Enrichment Analysis. Nine exposed samples exhibited proliferative lesions, while none of the non-exposed samples showed histological abnormalities, indicating an association of ionizing radiation exposure with histological abnormalities. Activated immune response signaling and deregulated metabolic processes were observed in irradiated tissue with normal histology compared to normal tissue from non-exposed samples. Proliferative lesions compared to corresponding normal tissues showed enrichment for mainly proliferation-associated gene sets. Consistently, proliferative lesion samples from exposed mice showed elevated proliferation-associated signaling and deregulated metabolic processes compared to normal samples from non-exposed mice. Our findings suggest that a molecular deregulation may be detectable in histologically normal thyroid tissues and in early proliferative lesions in the frame of multi-step progression from irradiated normal tissue to tumorous lesions.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Histology ; Thyroid Carcinogenesis ; Thyroid Neoplasia ; Thyroid Pathogenesis ; Transcriptomics
Language english
Publication Year 2021
HGF-reported in Year 2021
ISSN (print) / ISBN 1351-0088
e-ISSN 1479-6821
Journal Endocrine-Related Cancer
Quellenangaben Volume: 28, Issue: 3, Pages: 213-224 Article Number: , Supplement: ,
Publisher BioScientifica
Publishing Place Bristol
Reviewing status Peer reviewed
Institute(s) Translational Metabolic Oncology (IDC-TMO)
Institute of Radiation Biology (ISB)
Institute of Metabolism and Cell Death (MCD)
Institute of Experimental Genetics (IEG)
Research Unit Analytical Pathology (AAP)
Institute of Lung Health and Immunity (LHI)
CF Pathology & Tissue Analytics (CF-PTA)
POF-Topic(s) 30203 - Molecular Targets and Therapies
30202 - Environmental Health
30201 - Metabolic Health
30205 - Bioengineering and Digital Health
30505 - New Technologies for Biomedical Discoveries
Research field(s) Radiation Sciences
Genetics and Epidemiology
Enabling and Novel Technologies
Lung Research
PSP Element(s) G-501000-001
G-500200-001
G-506900-001
G-500600-001
G-500390-001
G-501600-014
A-630600-001
Grants Bundesministerium fur Bildung und Forschung (BMBF)
DOI 10.1530/ERC-21-0019
WOS ID WOS:000637371200008
Scopus ID 85104047187
PubMed ID 33608487
Erfassungsdatum 2021-05-11
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