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Römisch-Margl, W. ; Eisenreich, W.* ; Haase, I.* ; Bacher, A.* ; Fischer, M.*

2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthases of fungi and archaea.

FEBS J. 275, 4403-4414 (2008)
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The pathway of riboflavin (vitamin B2) biosynthesis is significantly different in archaea, eubacteria, fungi and plants. Specifically, the first committed intermediate, 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate, can either undergo hydrolytic cleavage of the position 2 amino group by a deaminase (in plants and most eubacteria) or reduction of the ribose side chain by a reductase (in fungi and archaea). We compare 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthases from the yeast Candida glabrata, the archaeaon Methanocaldococcus jannaschii and the eubacterium Aquifex aeolicus. All three enzymes convert 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate into 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate, as shown by 13C-NMR spectroscopy using [2,1',2',3',4',5'-13C6]2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate as substrate. The beta anomer was found to be the authentic substrate, and the alpha anomer could serve as substrate subsequent to spontaneous anomerisation. The M. jannaschii and C. glabrata enzymes were shown to be A-type reductases catalysing the transfer of deuterium from the 4(R) position of NADPH to the 1' (S) position of the substrate. These results are in agreement with the known three-dimensional structure of the M. jannaschii enzyme.
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Publication type Article: Journal article
Document type Scientific Article
Keywords 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthase; archaea; fungi; riboflavin biosynthesis; stereochemistry
Language english
Publication Year 2008
HGF-reported in Year 0
ISSN (print) / ISBN 1742-464X
e-ISSN 1742-4658
Journal FEBS Journal, The
Quellenangaben Volume: 275, Issue: 17, Pages: 4403-4414 Article Number: , Supplement: ,
Publisher Wiley
Publishing Place Oxford
Reviewing status Peer reviewed
Institute(s) Institute of Bioinformatics and Systems Biology (IBIS)
POF-Topic(s) 30505 - New Technologies for Biomedical Discoveries
Research field(s) Enabling and Novel Technologies
PSP Element(s) G-503700-001
PubMed ID 18671734
DOI 10.1111/j.1742-4658.2008.06586.x
Scopus ID 49349089962
Erfassungsdatum 2008-01-01
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