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A bio-conjugated fullerene as a subcellular-targeted and multifaceted phototheranostic agent.
Adv. Func. Mat. 31:2101527 (2021)
Fullerenes are candidates for theranostic applications because of their high photodynamic activity and intrinsic multimodal imaging contrast. However, fullerenes suffer from low solubility in aqueous media, poor biocompatibility, cell toxicity, and a tendency to aggregate. C70@lysozyme is introduced herein as a novel bioconjugate that is harmless to a cellular environment, yet is also photoactive and has excellent optical and optoacoustic contrast for tracking cellular uptake and intracellular localization. The formation, water-solubility, photoactivity, and unperturbed structure of C70@lysozyme are confirmed using UV-visible and 2D 1H, 15N NMR spectroscopy. The excellent imaging contrast of C70@lysozyme in optoacoustic and third harmonic generation microscopy is exploited to monitor its uptake in HeLa cells and lysosomal trafficking. Last, the photoactivity of C70@lysozyme and its ability to initiate cell death by means of singlet oxygen (1O2) production upon exposure to low levels of white light irradiation is demonstrated. This study introduces C70@lysozyme and other fullerene-protein conjugates as potential candidates for theranostic applications.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Fullerenes ; Lysozymes ; Multimodal Microscopy ; Optoacoustic Imaging ; Photodynamic Therapy ; Phototheranostic Agents
ISSN (print) / ISBN
1616-301X
e-ISSN
1616-3028
Journal
Advanced functional materials
Quellenangaben
Volume: 31,
Issue: 20,
Article Number: 2101527
Publisher
Wiley
Publishing Place
Weinheim
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Biological and Medical Imaging (IBMI)
Grants
Italian Ministry of Education, University and Research
Gottfried Wilhelm Leibniz Prize
Deutsche Forschungsgemeinschaft (DFG)
FIRC-AIRC fellowship for Italy
Italian Ministry of Education, University and Research (MIUR), SIR Programme
AIRC
Gottfried Wilhelm Leibniz Prize
Deutsche Forschungsgemeinschaft (DFG)
FIRC-AIRC fellowship for Italy
Italian Ministry of Education, University and Research (MIUR), SIR Programme
AIRC