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Miroshnikov, S.A.* ; Notova, S.V.* ; Skalnaya, M.G.* ; Sizova, E.A.* ; Marshinskaia, O.V.* ; Kazakova, T.V.* ; Skalny, A.V.* ; Michalke, B. ; Ajsuvakova, O.P.* ; Tinkov, A.A.*

Speciation of serum copper and zinc-binding high- and low-molecular mass ligands in dairy cows using HPLC-ICP-MS technique.

Biol. Trace Elem. Res. 200, 591-599 (2022)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
The objective of the present study was assessment of the major copper and zinc species in dairy cow blood serum using a hybrid high-performance liquid chromatography-inductively coupled plasma-mass spectrometry (HPLC-ICP-MS) technique. A total of seventeen 5–6-year-old female Simmental cows, cultivated in the Southern Ural region, were examined. Speciation of serum Cu and Zn was performed using chromatographic PerkinElmer Series 200 system equipped with Agilent Bio SEC-5 Column and docked with NexION 300D mass spectrometer. Analysis of serum Cu species revealed four major fractions containing 2.5% (A), 15.6% (B), 75.6% (C), and 11.9% (D) of total copper levels. The revealed fractions could be assigned to tetrameric and dimeric macroglobulin, ceruloplasmin, albumin, and low molecular mass (LMM) copper compounds, respectively. Minor fraction (E) containing <1% of total serum Cu levels may be represented by low-molecular mass Cu species. Speciation analysis also revealed four Zn fractions containing 6.3% (A), 16.9% (B), 71% (C), and 3% (D) of total Zn levels that may be attributed to zinc-bound tetrameric and dimeric macroglobulin, albumin, and Zn-amino acid compounds. Correlation analysis demonstrated that relative levels (%) of Zn-B (dimeric α2-macroglobulin), Zn-C (albumin), and Zn-D (LMM) fractions correlate inversely with Cu-A (monomeric α2-macroglobulin) (r = −0.600), Cu-D (albumin) (r = −0.696), and Cu-C (ceruloplasmin) (r = −0.652), respectively. The obtained data demonstrate the particular features of Zn and Cu transport in dairy cows that may be used for assessment of dietary status of trace elements. 63
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Albumin ; Ceruloplasmin ; Metallomics ; α2-macroglobulin
ISSN (print) / ISBN 0163-4984
e-ISSN 1559-0720
Journal Biological Trace Element Research
Quellenangaben Volume: 200, Issue: 2, Pages: 591-599 Article Number: , Supplement: ,
Publisher Springer
Publishing Place 999 Riverview Drive Suite 208, Totowa, Nj 07512 Usa
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Research Unit Analytical BioGeoChemistry (BGC)
Grants Russian Science Foundation
Ural Branch, Russian Academy of Sciences