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Del Castello, F.* ; Foresi, N.* ; Nejamkin, A.* ; Lindermayr, C. ; Buegger, F. ; Lamattina, L.* ; Correa-Aragunde, N.*

Cyanobacterial NOS expression improves nitrogen use efficiency, nitrogen-deficiency tolerance and yield in Arabidopsis.

Plant Sci. 307:110860 (2021)
Postprint DOI PMC
Open Access Green
Developing strategies to improve nitrogen (N) use efficiency (NUE) in plants is a challenge to reduce environmental problems linked to over-fertilization. The nitric oxide synthase (NOS) enzyme from the cyanobacteria Synechococcus PCC 7335 (SyNOS) has been recently identified and characterized. SyNOS catalyzes the conversion of arginine to citrulline and nitric oxide (NO), and then approximately 75 % of the produced NO is rapidly oxidized to nitrate by an unusual globin domain in the N-terminus of the enzyme. In this study, we assessed whether SyNOS expression in plants affects N metabolism, NUE and yield. Our results showed that SyNOS-expressing transgenic Arabidopsis plants have greater primary shoot length and shoot branching when grown under N-deficient conditions and higher seed production both under N-sufficient and N-deficient conditions. Moreover, transgenic plants showed significantly increased NUE in both N conditions. Although the uptake of N was not modified in the SyNOS lines, they showed an increase in the assimilation/remobilization of N under conditions of low N availability. In addition, SyNOS lines have greater N-deficiency tolerance compared to control plants. Our results support that SyNOS expression generates a positive effect on N metabolism and seed production in Arabidopsis, and it might be envisaged as a strategy to improve productivity in crops under adverse N environments.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Arabidopsis ; Nitric Oxide Synthase ; Nitrogen Deficiency Tolerance ; Nitrogen Use Efficiency ; Seed Yield ; Synechococcus Pcc 7335; Nitric-oxide Synthase; Alga Ostreococcus-tauri; Signaling Pathways; Nitrate Response; Genomic Analysis; Gene-expression; Thaliana; Protein; Growth; Roots
ISSN (print) / ISBN 0168-9452
e-ISSN 0168-9452
Journal Plant Science
Quellenangaben Volume: 307, Issue: , Pages: , Article Number: 110860 Supplement: ,
Publisher Elsevier
Publishing Place Elsevier House, Brookvale Plaza, East Park Shannon, Co, Clare, 00000, Ireland
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Biochemical Plant Pathology (BIOP)
Research Unit Environmental Simulation (BIOP-EUS)
Grants Agencia Nacional de Promocion Cientifica y Tecnologica (ANPCyT)
Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET)
Universidad Nacional de Mar del Plata (UNMdP), Argentina
German Academic Exchange Service (DAAD)-short term Fellowship