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Multiplexed serum antibody screening platform using virus extracts from endemic Coronaviridae and SARS-CoV-2.
ACS Infect. Dis. 7, 1596-1606 (2021)
The presence of antibodies against endemic coronaviruses has been linked to disease severity after SARS-CoV-2 infection. Assays capable of concomitantly detecting antibodies against endemic coronaviridae such as OC43, 229E, NL63, and SARS-CoV-2 may help to elucidate this question. We developed a serum screening platform using a bead-based Western blot system called DigiWest, capable of running hundreds of assays using microgram amounts of protein prepared directly from different viruses. Characterization of the immunoassay for detection of SARS-CoV-2 specific antibodies revealed a sensitivity of 90.3% and a diagnostic specificity of 98.1%. Concordance analysis with the SARS-CoV-2 immunoassays available by Roche, Siemens, and Euroimmun indicates comparable assay performances (Cohen's κ ranging from 0.8874 to 0.9508). Analogous assays for OC43, 229E, and NL63 were established and combined into one multiplex with the SARS-CoV-2 assay. Seroreactivity for different coronaviruses was detected with high incidence, and the multiplex assay was adapted for serum screening.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Covid-19 ; Luminex ; Sars-cov-2 ; Western Blot ; Endemic Coronavirus ; Serology; Immunity; Western
ISSN (print) / ISBN
2373-8227
e-ISSN
2373-8227
Journal
ACS infectious diseases
Quellenangaben
Volume: 7,
Issue: 6,
Pages: 1596-1606
Publisher
ACS
Publishing Place
1155 16th St, Nw, Washington, Dc 20036 Usa
Non-patent literature
Publications
Reviewing status
Peer reviewed
Grants
European Union