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Elimination of P. Aeruginosa in mice by treatment with chlorine, and the use of microbiological and PCR analyses.
Scand. J. Lab. Anim. Sci. 36, 355-361 (2009)
Unwanted micro-organisms in mice can jeopardize experimental protocols and should be eliminated if a colony becomes infected. The opportunistic bacterium Pseudomonas aeruginosa has been shown to cause infection in mice. In order to eliminate this bacterium from a specific-pathogen-free full barrier mouse area at our animal facility we treated mice with 7 ppm chlorine for 7 weeks and then with 10 ppm chlorine in the drinking water. The P. aeruginosa status of mice was examined by agar culture and PCR. The results show that 6 months after commencement of the treatment the colony was free of P. aeruginosa.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Pseudomonas-aerginosa; Cyxlophosphamide; Recommendations; Colonies; Samples
ISSN (print) / ISBN
0901-3393
Quellenangaben
Volume: 36,
Issue: 4,
Pages: 355-361
Publisher
Scandinavian Federation for Laboratory Animal Science
Publishing Place
Soborg, Denmark
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
CF Comparative Medicine (CF-Comparative Medicine)