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Open Access Green as soon as Postprint is submitted to ZB.
Bisphenol F and bisphenol S promote lipid accumulation and adipogenesis in human adipose-derived stem cells.
Food Chem. Toxicol. 152:112216 (2021)
Bisphenol F (BPF) and bisphenol S (BPS) are increasingly used as substitutes for bisphenol A (BPA), an endocrine disrupting chemical (EDC) with obesogenic activity. We investigated the in vitro effects of BPS and BPF on the adipogenesis of human adipose-derived stem cells (hASCs) exposed to different doses (0.01, 0.1, 1, 10 and 25 μM), stopping the adipogenic process at 7 or 14 days. Intracellular lipid accumulation was quantified by the Oil Red O assay, gene expression of peroxisome proliferator-activated receptor gamma (PPARγ), CCAT/enhancer-binding protein (C/EBPα), lipoprotein-lipase (LPL) and fatty acid binding protein 4 (FABP4), by quantitative real-time polymerase chain reaction (qRT-PCR) and protein levels by Western Blot. hASCs with BPF or BPS produced a linear dose-response increase in intracellular lipid accumulation and in gene expression of the adipogenic markers, confirmed by protein levels. Co-treatment ICI 182,780 significantly inhibited BPF- but not BPS-induced lipid accumulation. Given the affinity of bisphenols for diverse nuclear receptors, their obesogenic effects may result from a combination of pathways rather than a single mechanism. Further research is warranted on the manner in which chemicals interfere with adipogenic differentiation. To our best knowledge, this report shows for the first time the obesogenic potential of BPF in hASCs.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Adipogenesis ; Bisphenol A (bpa) ; Bisphenol F (bpf) ; Bisphenol S (bps) ; Endocrine Disruptors ; Obesity; Disrupting Chemicals; Human Exposure; In-vitro; Differentiation; Obesity; Alternatives; Health; Tissue; Preadipocytes; Urinary
ISSN (print) / ISBN
0278-6915
e-ISSN
0278-6915
Journal
Food and Chemical Toxicology
Quellenangaben
Volume: 152,
Article Number: 112216
Publisher
Elsevier
Publishing Place
The Boulevard, Langford Lane, Kidlington, Oxford Ox5 1gb, England
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Diabetes and Obesity (IDO)
Grants
Spanish Ministry of Education
Institute of Health Carlos III - European Regional Development Fund/FEDER
Biomedical Research Networking Center-CIBER de Epidemiologia y Salud Publica (CIBERESP) of the Institute of Health Carlos III
European Union
Institute of Health Carlos III - European Regional Development Fund/FEDER
Biomedical Research Networking Center-CIBER de Epidemiologia y Salud Publica (CIBERESP) of the Institute of Health Carlos III
European Union