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Muller, L.A.H.* ; Ballhausen, M.B.* ; Andrade Linares, D.R. ; Pinek, L.* ; Golubeva, P.* ; Rillig, M.C.*

Fungus-bacterium associations are widespread in fungal cultures isolated from a semi-arid natural grassland in Germany.

FEMS Microbiol. Ecol. 97:fiab059 (2021)

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	Open Access Green

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We report on a study that aimed at establishing a large soil-fungal culture collection spanning a wide taxonomic diversity and systematically screening the collection for bacterial associations. Fungal cultures were isolated from soil samples obtained from a natural grassland in eastern Germany and bacterial associations were assessed by PCR-amplification and sequencing of bacterial 16S rRNA. In addition, intraspecies genetic diversities of a subset of the isolated species were estimated by double-digest restriction associated DNA sequencing. A total of 688 fungal cultures, representing at least 106 fungal species from 36 different families, were obtained and even though clonal isolates were identified in almost all fungal species subjected to ddRAD-seq, relatively high genetic diversities could be observed in some of the isolated species. Sixty-nine % of the fungal isolates in our collection were found to be associated with bacteria and the most commonly identified bacterial genera were Pelomonas, Enterobacter and Burkholderia. Our results indicate that bacterial associations commonly occur in soil fungi, even if antibiotics are being applied during the isolation process, and provide a basis for the use of our culture collection in ecological experiments that want to acknowledge the importance of intraspecies genetic diversity.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Bacteria ; Fungal-bacterial Interaction ; Soil Fungi ; Ddrad Sequencing; Sp Nov.; Mycorrhizal; Identification; Diversity; Sequence; Hyphae

ISSN (print) / ISBN 0168-6496

e-ISSN 1574-6941

Journal FEMS Microbiology Ecology

Quellenangaben Volume: 97, Issue: 5, Article Number: fiab059

Publisher Wiley

Publishing Place Oxford

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Research Unit Comparative Microbiome Analysis (COMI)

Grants European Research Council