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Observing islet function and islet-immune cell interactions in live pancreatic tissue slices.
J. Vis. Exp. 2021:e62207 (2021)
Live pancreatic tissue slices allow for the study of islet physiology and function in the context of an intact islet microenvironment. Slices are prepared from live human and mouse pancreatic tissue embedded in agarose and cut using a vibratome. This method allows for the tissue to maintain viability and function in addition to preserving underlying pathologies such as type 1 (T1D) and type 2 diabetes (T2D). The slice method enables new directions in the study of the pancreas through the maintenance of the complex structures and various intercellular interactions that comprise the endocrine and exocrine tissues of the pancreas. This protocol demonstrates how to perform staining and time-lapse microscopy of live endogenous immune cells within pancreatic slices along with assessments of islet physiology. Further, this approach can be refined to discern immune cell populations specific for islet cell antigens using major histocompatibility complex-multimer reagents.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
In-situ; Langerhans; Mechanisms
ISSN (print) / ISBN
1940-087X
e-ISSN
1940-087X
Quellenangaben
Volume: 2021,
Issue: 170,
Article Number: e62207
Publisher
JoVE
Publishing Place
1 Alewife Center, Ste 200, Cambridge, Ma 02140 Usa
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute for Pancreatic Beta Cell Research (IPI)
Grants
Leona M. & Harry B. Helmsley Charitable Trust
JDRF
NIH
JDRF
NIH