Home
Deutsch
Advanced Search
Browse by ...
Publication overview
Contact persons
Help
Publ. Version/Full Text
DOI
PMC
 |
Open Access Gold (Paid Option) |
|
as soon as is submitted to ZB.
Brief Guide: Experimental strategies for high-quality hit selection from small-molecule screening campaigns.
SLAS Discov. 26, 851-854 (2021)
Small-molecule screening is a powerful approach to identify modulators of either specific biological targets or cellular pathways with phenotypic endpoints. A myriad of assay technologies are available to assess the activity of enzymes, monitor protein-protein interactions, measure transcription factor activity in reporter assays, or detect cellular features and activities using high-content imaging. A common challenge during small-molecule screening is, however, the presence of hit compounds generating assay interference, thereby producing false-positive hits. Thus, efforts are needed to uncover such interferences to prioritize high-quality hits for further analysis. This process encompasses (1) computational approaches to flag undesirable compounds, and (2) the use of experimental approaches like counter, orthogonal, and cellular fitness screens to identify and eliminate artifacts. In this brief guide, we provide an overview for first-time users, highlighting experimental screening strategies to prioritize high-quality bioactive hits from high-throughput screening/high-content screening (HTS/HCS) campaigns.
Altmetric
Additional Metrics?
Edit extra informations
Login
Publication type
Article: Journal article
Document type
Review
Keywords
Cellular Health ; Counter Assay ; False-positive Exclusion ; High-content Screening ; High-throughput Screening ; Hit Triaging ; Orthogonal Assay; Frequent Hitters; Assay; Identification
ISSN (print) / ISBN
2472-5552
e-ISSN
2472-5560
Journal
SLAS Discovery
Quellenangaben
Volume: 26,
Issue: 7,
Pages: 851-854
Publisher
Sage
Publishing Place
Thousand Oaks, Calif.
Non-patent literature
Publications
Reviewing status
Peer reviewed