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Salzer, L. ; Witting, M.

Quo vadis Caenorhabditis elegans metabolomics - A review of current methods and applications to explore metabolism in the nematode.

Metabolites 11:284 (2021)
Publ. Version/Full Text DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Metabolomics and lipidomics recently gained interest in the model organism Caenorhabditis elegans (C. elegans). The fast development, easy cultivation and existing forward and reverse genetic tools make the small nematode an ideal organism for metabolic investigations in development, aging, different disease models, infection, or toxicology research. The conducted type of analysis is strongly depending on the biological question and requires different analytical approaches. Metabolomic analyses in C. elegans have been performed using nuclear magnetic resonance (NMR) spectroscopy, direct infusion mass spectrometry (DI-MS), gas-chromatography mass spectrometry (GC-MS) and liquid chromatography mass spectrometry (LC-MS) or combinations of them. In this review we provide general information on the employed techniques and their advantages and disadvantages in regard to C. elegans metabolomics. Additionally, we reviewed different fields of application, e.g., longevity, starvation, aging, development or metabolism of secondary metabolites such as ascarosides or maradolipids. We also summarised applied bioinformatic tools that recently have been used for the evaluation of metabolomics or lipidomics data from C. elegans. Lastly, we curated metabolites and lipids from the reviewed literature, enabling a prototypic collection which serves as basis for a future C. elegans specific metabolome database.
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Publication type Article: Journal article
Document type Review
Corresponding Author
Keywords Caenorhabditis Elegans ; Nmr ; Lipidomics ; Mass-spectrometry ; Metabolomics; Polyunsaturated Fatty-acids; Functional Genomic Analysis; Small-molecule Signals; High-resolution; C. Elegans; Life-span; Liquid-chromatography; Dietary Restriction; Gas-chromatography; Mass-spectrometry
ISSN (print) / ISBN 2218-1989
e-ISSN 2218-1989
Journal Metabolites
Quellenangaben Volume: 11, Issue: 5, Pages: , Article Number: 284 Supplement: ,
Publisher MDPI
Publishing Place St Alban-anlage 66, Ch-4052 Basel, Switzerland
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Research Unit Analytical BioGeoChemistry (BGC)
CF Metabolomics & Proteomics (CF-MPC)
Grants Deutsche Forschungsgemeinschaft (DFG, German Research foundation)