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Cosco, E. ; Arus, B.A. ; Spearman, A.L.* ; Atallah, T.L.* ; Lim, I.* ; Leland, O.S.* ; Caram, J.R.* ; Bischof, T.S. ; Bruns, O.T. ; Sletten, E.M.*

Bright chromenylium polymethine dyes enable fast, four-color in vivo imaging with shortwave infrared detection.

J. Am. Chem. Soc. 143, 6836-6846 (2021)

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	Open Access Gold (Paid Option)

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Optical imaging within the shortwave infrared (SWIR, 1000-2000 nm) region of the electromagnetic spectrum has enabled high-resolution and high-contrast imaging in mice, non-invasively. Polymethine dyes, with their narrow absorption spectra and high absorption coefficients, are optimal probes for fast and multiplexed SWIR imaging. Here, we expand upon the multiplexing capabilities in SWIR imaging by obtaining brighter polymethine dyes with varied excitation wavelengths spaced throughout the near-infrared (700-1000 nm) region. Building on the flavylium polymethine dye scaffold, we explored derivatives with functional group substitution at the 2-position, deemed chromenylium polymethine dyes. The reported dyes have reduced nonradiative rates and enhanced emissive properties, enabling non-invasive imaging in mice in a single color at 300 fps and in three colors at 100 fps. Combined with polymethine dyes containing a red-shifted julolidine flavylium heterocycle and indocyanine green, distinct channels with well-separated excitation wavelengths provide non-invasive video-rate in vivo imaging in four colors.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Cyanine; Fluorophores; Surgery

ISSN (print) / ISBN 0002-7863

e-ISSN 1520-5126

Journal Journal of the American Chemical Society

Quellenangaben Volume: 143, Issue: 18, Pages: 6836-6846

Publisher American Chemical Society (ACS)

Publishing Place 1155 16th St, Nw, Washington, Dc 20036 Usa

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Helmholtz Pioneer Campus (HPC)

Grants UCLA
DOE
NSF
Foote Family
Alfred P. Sloan Foundation
Emmy-Noether-Program of DFG
Helmholtz Pioneer Campus Institute for Biomedical Engineering
NIH