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Yazgili, A.S. ; Meul, T. ; Welk, V. ; Semren, N. ; Kammerl, I.E. ; Meiners, S.

In-gel proteasome assay to determine the activity, amount, and composition of proteasome complexes from mammalian cells or tissues.

STAR Protoc. 2:100526 (2021)

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	Open Access Gold

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This protocol describes an easy and reliable in-gel proteasome assay to quantify the activity and composition of different proteasome complexes in cells and tissues. The assay works well with limited amounts of total cell protein lysates. Although this assay is optimized specifically for the proteasome chymotrypsin-like activity, it can be expanded to other proteasome activities as well. Using antibodies that detect distinct proteasome subunits or regulators, we can determine the composition and relative quantity of active proteasome complexes. For complete details on the use and execution of this protocol, please refer to Meul et al. (2020).

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Antibody ; Cell Biology ; Molecular Biology ; Protein Biochemistry

e-ISSN 2666-1667

Journal STAR Protocols

Quellenangaben Volume: 2, Issue: 2, Article Number: 100526

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Lung Health and Immunity (LHI)