Home
Deutsch
Advanced Search
Browse by ...
Publication overview
Contact persons
Help
Publ. Version/Full Text
DOI
PMC
 |
Open Access Gold |
|
as soon as is submitted to ZB.
Diamond nanoparticles-porphyrin mthpp conjugate as photosensitizing platform: Cytotoxicity and antibacterial activity.
Nanomaterials 11:1393 (2021)
Conjugation of photosensitizers (PS) with nanoparticles has been largely used as a strategy to stabilize PS in the biological medium resulting in photosensitizing nanoparticles of enhanced photoactivity. Herein, (Meso-5, 10, 15, 20-tetrakis (3-hydroxyphenyl) phorphyryn (mTHPP) was conjugated with diamond nanoparticles (ND) by covalent bond. Nanoconjugate ND-mTHPP showed suitable stability in aqueous suspension with 58 nm of hydrodynamic diameter and Zeta potential of −23 mV. The antibacterial activity of ND-mTHPP was evaluated against Escherichia coli for different incubation times (0–24 h). The optimal activity was observed after 2 h of incubation and irradiation (660 nm; 51 J/cm ) performed right after the addition of ND-mTHPP (100 µg/mL) to the bacterial suspension. The inhibitory activity was 56% whereas ampicillin at the same conditions provided only 14% of bacterial growth inhibition. SEM images showed agglomerate of ND-mTHPP adsorbed on the bacterial cell wall, suggesting that the antimicrobial activity of ND-mTHPP was afforded by inducing membrane damage. Cytotoxicity against murine embryonic fibroblast cells (MEF) was also evaluated and ND-mTHPP was shown to be noncytotoxic since viability of cells cultured for 24 h in the presence of the nanoconjugate (100 µg/mL) was 78%. Considering the enhanced antibacterial activity and the absence of cytotoxic effect, it is possible to consider the ND-mTHPP nanoconjugate as promising platform for application in antimicrobial photodynamic therapy (aPDT). 2
Altmetric
Additional Metrics?
Edit extra informations
Login
Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Drug-resistance ; Nanoconjugate ; Photodynamic Antimicrobial Therapy (apdt) ; Photosensitizers (ps); Photodynamic Therapy; Photophysicochemical Properties; Antimicrobial Activity; Surface Modification; Nanodiamond; Cancer; Functionalization; Phthalocyanine; Fluorescence; Metabolites
ISSN (print) / ISBN
2079-4991
e-ISSN
2079-4991
Journal
Nanomaterials
Quellenangaben
Volume: 11,
Issue: 6,
Article Number: 1393
Publisher
MDPI
Publishing Place
Basel
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Medicinal Chemistry (IMC)
Grants
Fundacao de Amparo ao Ensino e Pesquisa do Estado de Sao Paulo
Fundacao de Amparo ao Ensino e Pesquisa do Estado de Sao Paulo