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Open Access Green as soon as Postprint is submitted to ZB.
Isolation and Eeectron microscopic analysis of liver cancer cell mitochondria.
In: Mitochondrial Medicine. Berlin [u.a.]: Springer, 2021. 277-287 (Methods Mol. Biol. ; 2277)
Isolation of mitochondria is a crucial method for examining molecular details of this organelle's manifold functions. Historically, mitochondrial isolations required large amounts of sample material which impeded their isolation from cultured cells. We have therefore developed a method allowing for controlled and reproducible isolation of intact and functional mitochondria from diverse cell types in culture. Here we provide a methodological update of this approach together with a protocol for the subsequent analysis of such isolated mitochondria by electron microscopy. Combining the isolation procedure with this powerful imaging method can reveal ultrastructural mitochondrial peculiarities in disease settings that might not be evident in intact cells and allows for assessment of mitochondrial membrane integrity and sample purity.
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Publication type
Article: Edited volume or book chapter
Keywords
Balch Homogenizer ; Cell Culture ; Electron Microscopy ; Image Analysis ; Mitochondria
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Book Volume Title
Mitochondrial Medicine
Journal
Methods in Molecular Biology
Quellenangaben
Volume: 2277,
Pages: 277-287
Publisher
Springer
Publishing Place
Berlin [u.a.]
Non-patent literature
Publications
Reviewing status
Peer reviewed