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Residual pluripotency is required for inductive germ cell segregation.
EMBO Rep.:e52553 (2021)
Fine-tuned dissolution of pluripotency is critical for proper cell differentiation. Here we show that the mesodermal transcription factor, T, globally affects the properties of pluripotency through binding to Oct4 and to the loci of other pluripotency regulators. Strikingly, lower T levels coordinately affect naïve pluripotency, thereby directly activating the germ cell differentiation program, in contrast to the induction of germ cell fate of primed models. Contrary to the effect of lower T levels, higher T levels more severely affect the pluripotency state, concomitantly enhancing the somatic differentiation program and repressing the germ cell differentiation program. Consistent with such in vitro findings, nascent germ cells in vivo are detected in the region of lower T levels at the posterior primitive streak. Furthermore, T and core pluripotency regulators co-localize at the loci of multiple germ cell determinants responsible for germ cell development. In conclusion, our findings indicate that residual pluripotency establishes the earliest and fundamental regulatory mechanism for inductive germline segregation from somatic lineages.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
T(brachyury) ; Cell Fate Determination ; Germ Cells ; Mesoderm ; Pluripotency; Epiblast Stem-cells; Mouse Embryos; Ground-state; Mesoderm Formation; In-vitro; Specification; Lineage; Brachyury; Differentiation; Subpopulations
ISSN (print) / ISBN
1469-221X
e-ISSN
1469-3178
Journal
EMBO Reports
Quellenangaben
Article Number: e52553
Publisher
EMBO Press
Publishing Place
111 River St, Hoboken 07030-5774, Nj Usa
Non-patent literature
Publications
Reviewing status
Peer reviewed
Grants
Max-Planck-Society