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Esteve-Codina, A.* ; Hofer, T.P. ; Burggraf, D. ; Heiss-Neumann, M.S. ; Gesierich, W. ; Boland, A.* ; Olaso, R.* ; Bihoreau, M.T.* ; Deleuze, J.F.* ; Möller, W. ; Schmid, O. ; Soler Artigas, M.* ; Renner, K.* ; Hohlfeld, J.M.* ; Welte, T.* ; Fuehner, T.* ; Jerrentrup, L.* ; Koczulla, A.R.* ; Greulich, T.* ; Prasse, A.* ; Müller-Quernheim, J.* ; Gupta, S.* ; Brightling, C.* ; Subramanian, D.R.* ; Parr, D.G.* ; Kolsum, U.* ; Gupta, V.* ; Barta, I.* ; Döme, B.* ; Strausz, J.* ; Stendardo, M.* ; Piattella, M.* ; Boschetto, P.* ; Korzybski, D.* ; Gorecka, D.* ; Nowinski, A.* ; Dabad, M.* ; Fernández-Callejo, M.* ; Endesfelder, D. ; zu Castell, W. ; Hiemstra, P.S.* ; Venge, P.* ; Nößner, E. ; Griebel, T.* ; Heath, S.* ; Singh, D.* ; Gut, I.* ; Ziegler-Heitbrock, L.

Gender specific airway gene expression in COPD sub-phenotypes supports a role of mitochondria and of different types of leukocytes.

Sci. Rep. 11:12848 (2021)
Publ. Version/Full Text DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Chronic obstructive pulmonary disease (COPD) is a destructive inflammatory disease and the genes expressed within the lung are crucial to its pathophysiology. We have determined the RNAseq transcriptome of bronchial brush cells from 312 stringently defined ex-smoker patients. Compared to healthy controls there were for males 40 differentially expressed genes (DEGs) and 73 DEGs for females with only 26 genes shared. The gene ontology (GO) term "response to bacterium" was shared, with several different DEGs contributing in males and females. Strongly upregulated genes TCN1 and CYP1B1 were unique to males and females, respectively. For male emphysema (E)-dominant and airway disease (A)-dominant COPD (defined by computed tomography) the term "response to stress" was found for both sub-phenotypes, but this included distinct up-regulated genes for the E-sub-phenotype (neutrophil-related CSF3R, CXCL1, MNDA) and for the A-sub-phenotype (macrophage-related KLF4, F3, CD36). In E-dominant disease, a cluster of mitochondria-encoded (MT) genes forms a signature, able to identify patients with emphysema features in a confirmation cohort. The MT-CO2 gene is upregulated transcriptionally in bronchial epithelial cells with the copy number essentially unchanged. Both MT-CO2 and the neutrophil chemoattractant CXCL1 are induced by reactive oxygen in bronchial epithelial cells. Of the female DEGs unique for E- and A-dominant COPD, 88% were detected in females only. In E-dominant disease we found a pronounced expression of mast cell-associated DEGs TPSB2, TPSAB1 and CPA3. The differential genes discovered in this study point towards involvement of different types of leukocytes in the E- and A-dominant COPD sub-phenotypes in males and females.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Obstructive Pulmonary-disease; Oxidative Stress; Gro-alpha; Emphysema; Epithelium
ISSN (print) / ISBN 2045-2322
e-ISSN 2045-2322
Journal Scientific Reports
Quellenangaben Volume: 11, Issue: 1, Pages: , Article Number: 12848 Supplement: ,
Publisher Nature Publishing Group
Publishing Place London
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) CF Immunoanalytics (IMA)
Lung Health and Immunity (LHI)
Institute of Computational Biology (ICB)
Strategy and Digitalization (DIG)
Grants European Commission