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Allergen extract- and component-based diagnostics in children of the ALLIANCE asthma cohort.
Clin. Exp. Allergy 51, 1331-1345 (2021)
BACKGROUND: Current in vitro allergen-specific IgE (sIgE) detection assays measure IgE against allergen extracts or molecules in a single- or multiplex approach. Direct comparisons of the performance of such assays among young children with common presentations of allergic diseases regardless of sensitization status are largely missing. OBJECTIVES: The aim of this study was a comparison of the analytical and diagnostic performance for common clinical questions of three commonly used technologies which rely upon different laboratory methodologies among children of the All Age Asthma (ALLIANCE) cohort (clinicaltrials.gov: NCT02496468). METHODS: Sera from 106 pediatric study participants (mean age 4 years) were assessed for the presence of sIgE by means of the ImmunoCAP™ sx1 and fx5 mixes, the ImmunoCAP ISAC™ 112 microarray, and a Euroline™ panel. RESULTS: Total and negative concordance were high (>82% - >89%), while positive concordance varied considerably (0-100%) but was also >50% for the most common sensitizations analyzed (house dust mite and birch). All three test systems showed good sensitivity and specificity (AUC consistently > 0.7). However, no significant differences with regards to identifying sIgE sensitizations associated with symptoms in children with suspected pollen- or dust-triggered wheeze or presenting with symptoms of allergic rhinoconjunctivitis or food allergy were detected. Extending the number of allergens did not change the similar performance of the three assay systems. CONCLUSION AND CLINICAL RELEVANCE: Among young children, the three sIgE assays showed good analytical and diagnostic concordance. Our results caution that the identification of larger numbers of sensitizations by more comprehensive multiplex approaches may not improve clinical utility of sIgE testing in this age group.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Allergen Sige ; Analytical Performance ; Diagnostic Performance ; In Vitro Allergy Diagnosis ; Molecular Allergology; Predictive-value; Ige; Isac; Tool; Sensitization; Childhood; Immunocap; Molecules; Rhinitis; Tests
ISSN (print) / ISBN
0954-7894
e-ISSN
1365-2222
Journal
Clinical & Experimental Allergy
Quellenangaben
Volume: 51,
Issue: 10,
Pages: 1331-1345
Publisher
Wiley
Publishing Place
111 River St, Hoboken 07030-5774, Nj Usa
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Asthma and Allergy Prevention (IAP)
Grants
Foundation for Pathobiochemistry and Molecular Diagnostics
German Center for Lung Research (DZL)
University Hospital Giebetaen and Marburg (UKGM)
Deutsche Forschungsgemeinschaft (DFG)
Thermo Fisher Scientific, Sweden
Universities Giessen and Marburg Lung Center (UGMLC)
German Center for Lung Research (DZL)
University Hospital Giebetaen and Marburg (UKGM)
Deutsche Forschungsgemeinschaft (DFG)
Thermo Fisher Scientific, Sweden
Universities Giessen and Marburg Lung Center (UGMLC)