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Shahryari, A. ; Burtscher, I. ; Nazari, Z.* ; Lickert, H.

Engineering gene therapy: Advances and barriers.

Adv. Therap. 4:2100040 (2021)
Publ. Version/Full Text DOI
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
Currently, 33 gene-therapy drugs/products have been approved in the clinic. Over 3000 completed and ongoing clinical gene therapy trials have been reported worldwide. The development/maturation of tools for gene manipulation and gene delivery, as well as molecular advances in the diagnosis of genetic diseases, have played a central role in gene therapy, which have greatly revolutionized the field. Versatile and diverse genetic tools for gene manipulations and deliveries, with the possibility of short and long-term effects, are vital advantages of gene therapy tools, paving the road for the development of new therapies. However, efficacy and safety concerns, immune system responses, laborious approaches for developing and manufacturing, unknown gene-therapy drug interactions with the host and the high cost of drugs/products, are significant barriers in gene therapy. Here, the authors review the attempts for engineering of the gene manipulations that have been undertaken in the last three decades and used in clinical trials focusing on 1) gene-editing platforms, 2) viral gene delivery systems, and 3) nonviral gene tools. In this comprehensive review, the principles of these gene manipulation tools as well as advances and barriers for their application in modern therapies are discussed. Furthermore, trends and future directions in gene therapy are discussed.
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Publication type Article: Journal article
Document type Review
Corresponding Author
Keywords Advances ; Barriers ; Gene Therapy ; Gene-editing Platforms ; Genetic Tools ; Nonviral Tools ; Viral Vectors
ISSN (print) / ISBN 2366-3987
e-ISSN 2366-3987
Journal Advanced Therapeutics
Quellenangaben Volume: 4, Issue: 9, Pages: , Article Number: 2100040 Supplement: ,
Publisher Wiley
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Diabetes and Regeneration Research (IDR)