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Kirstein, A. ; Schilling, D. ; Combs, S.E. ; Schmid, T.E.

Lomeguatrib increases the radiosensitivity of mgmt unmethylated human glioblastoma multiforme cell lines.

Int. J. Mol. Sci. 22:6781 (2021)

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	Open Access Gold

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Background: Treatment resistance of glioblastoma multiforme to chemo-and radiotherapy remains a challenge yet to overcome. In particular, the O6-methylguanine-DNA-methyltransferase (MGMT) promoter unmethylated patients have only little benefit from chemotherapy treatment using temozolomide since MGMT counteracts its therapeutic efficacy. Therefore, new treatment options in radiotherapy need to be developed to inhibit MGMT and increase radiotherapy response. Methods: Lomeguatrib, a highly specific MGMT inhibitor, was used to inactivate MGMT protein in vitro. Radiosensitivity of established human glioblastoma multiforme cell lines in combination with lomeguatrib was investigated using the clonogenic survival assay. Inhibition of MGMT was analyzed using Western Blot. Cell cycle distribution and apoptosis were investigated to determine the effects of lomeguatrib alone as well as in combination with ionizing radiation. Results: Lomeguatrib significantly decreased MGMT protein and reduced radiation-induced G2/M arrest. A radiosensitizing effect of lomeguatrib was observed when administered at 1 µM and increased radioresistance at 20 µM. Conclusion: Low concentrations of lomeguatrib elicit radiosensitization, while high concentrations mediate a radioprotective effect.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Glioblastoma ; Lomeguatrib ; Mgmt ; Radiosensitivity ; Radiotherapy; Temozolomide Plus O-6-benzylguanine; Central-nervous-system; Recurrent; Alkyltransferase; Inactivation; Resistance; Cycle; Dna; Repair; Glioma

ISSN (print) / ISBN 1422-0067

e-ISSN 1661-6596

Journal International Journal of Molecular Sciences

Quellenangaben Volume: 22, Issue: 13, Article Number: 6781

Publisher MDPI

Publishing Place Basel

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Radiation Medicine (IRM)

Grants Medical Faculty of TUM
German Consortium for Translational Cancer Research