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Degtyaruk, O. ; Mc Larney, B. ; Deán-Ben, X.L.* ; Shoham, S.* ; Razansky, D.

Optoacoustic visualization of GCaMP6f labeled deep brain activity in a murine intracardiac perfusion model.

Proc. SPIE 11629:116292D (2021)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
The inability to directly visualize large-scale neural dynamics across the entire mammalian brain in the millisecond temporal resolution regime is among the main limitations of existing neuroimaging methods. Recent advances in optoacoustic imaging systems have led to the establishment of this technology as an alternative method for real-time deep-tissue observations. Particularly, functional optoacoustic neurotomography (FONT) has recently been suggested for three-dimensional imaging of both direct calcium activity and cerebral hemodynamic parameters in rodents. However, the lack of suitable calcium indicators featuring optical absorption peaks within the so-called near-infrared window has hampered the applicability of FONT for imaging neuronal activity deep within the mammalian brain. To surmount this challenge, we developed and validated an intracardially perfused murine brain model labelled with genetically encoded calcium indicator GCaMP6f that closely simulates in vivo conditions. Penetration of light through skull and skin is greatly facilitated after blood is substituted by artificial cerebrospinal fluid (ACSF). The new preparation enabled here the observation of stimulus-evoked calcium dynamics within the mouse brain at penetration depths and spatio-temporal resolution scales not attainable with other neuroimaging techniques.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Calcium Dynamics ; Functional Neuroimaging ; Gcamp6f ; Intracardiac Perfusion ; Optoacoustics
ISSN (print) / ISBN 0277-786X
e-ISSN 1996-756X
Journal Proceedings of SPIE
Quellenangaben Volume: 11629, Issue: , Pages: , Article Number: 116292D Supplement: ,
Publisher SPIE
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Biological and Medical Imaging (IBMI)
Grants National Institutes of Health (NIH)
EC | European Research Council (ERC)