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Fusarium head blight resistance in European winter wheat: Insights from genome-wide transcriptome analysis.
BMC Genomics 22:470 (2021)
Background: Fusarium head blight (FHB) is a devastating disease of wheat worldwide. Resistance to FHB is quantitatively controlled by the combined effects of many small to medium effect QTL. Flowering traits, especially the extent of extruded anthers, are strongly associated with FHB resistance. Results: To characterize the genetic basis of FHB resistance, we generated and analyzed phenotypic and gene expression data on the response to Fusarium graminearum (Fg) infection in 96 European winter wheat genotypes, including several lines containing introgressions from the highly resistant Asian cultivar Sumai3. The 96 lines represented a broad range in FHB resistance and were assigned to sub-groups based on their phenotypic FHB severity score. Comparative analyses were conducted to connect sub-group-specific expression profiles in response to Fg infection with FHB resistance level. Collectively, over 12,300 wheat genes were Fusarium responsive. The core set of genes induced in response to Fg was common across different resistance groups, indicating that the activation of basal defense response mechanisms was largely independent of the resistance level of the wheat line. Fg-induced genes tended to have higher expression levels in more susceptible genotypes. Compared to the more susceptible non-Sumai3 lines, the Sumai3-derivatives demonstrated higher constitutive expression of genes associated with cell wall and plant-type secondary cell wall biogenesis and higher constitutive and Fg-induced expression of genes involved in terpene metabolism. Gene expression analysis of the FHB QTL Qfhs.ifa-5A identified a constitutively expressed gene encoding a stress response NST1-like protein (TraesCS5A01G211300LC) as a candidate gene for FHB resistance. NST1 genes are key regulators of secondary cell wall biosynthesis in anther endothecium cells. Whether the stress response NST1-like gene affects anther extrusion, thereby affecting FHB resistance, needs further investigation. Conclusion: Induced and preexisting cell wall components and terpene metabolites contribute to resistance and limit fungal colonization early on. In contrast, excessive gene expression directs plant defense response towards programmed cell death which favors necrotrophic growth of the Fg pathogen and could thus lead to increased fungal colonization.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Cell Wall Modification ; Fhb1 ; Fusarium Graminearum ; Nst1 ; Qfhs.ifa-5a ; Rna-seq ; Sumai-3 ; Terpene ; Triticum Aestivum; Plant Defense; Spring Wheat; Cell-death; Deoxynivalenol; Graminearum; Mechanisms; Responses; Barley; Fhb1; Qtls
ISSN (print) / ISBN
1471-2164
e-ISSN
1471-2164
Journal
BMC Genomics
Quellenangaben
Volume: 22,
Issue: 1,
Article Number: 470
Publisher
BioMed Central
Publishing Place
Campus, 4 Crinan St, London N1 9xw, England
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Research Unit Environmental Simulation (BIOP-EUS)
Research Unit Plant Genome and Systems Biology (PGSB)
Institute of Environmental Medicine (IEM)
Research Unit Plant Genome and Systems Biology (PGSB)
Institute of Environmental Medicine (IEM)
Grants
Deutsche Forschungsgemeinschaft (DFG)
Austrian Science Fund (FWF)
Austrian Science Fund (FWF)