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Sabrautzki, S. ; Miller, M. ; Kague, E.* ; Brielmeier, M.

Welfare assessment of adult laboratory zebrafish: A practical guide.

Zebrafish 18, 282-292 (2021)
Postprint DOI PMC
Open Access Green
Teleost fish such as Danio rerio (zebrafish) have been successfully used in biomedical research since decades. Genetically altered fish lines obtained by state-of-the-art genetic technologies are serving as well-known model organisms. In Europe, following Directive 2010/63/EU, generation, breeding, and husbandry of new genetically altered lines of laboratory animals require governmental state approval in case pain, suffering, distress, or long-lasting harm to the offspring derived by breeding of these lines cannot be excluded. The identification and assessment of pain, distress, or harm, according to a severity classification of mild, moderate, severe, or humane endpoint, became a new challenging task for all scientists, animal technicians, and veterinarians for daily work with laboratory zebrafish. In this study, we describe the performance of the assessment of welfare parameters of selected pathologic phenotypes and abnormalities frequently found in laboratory fish facilities based on veterinary, biological, and physiological aspects by using a dedicated score sheet. In a colony of zebrafish, we evaluated the frequency of genotype-independent abnormalities observed within 3 years. We give examples for severity classification and measures once an abnormality has been identified according to the 3Rs (Replacement, Reduction and Refinement).
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Laboratory Fish ; Score Sheet ; Welfare Assessment ; Zebrafish; Danio-rerio; Craniofacial Development; Genetic-analysis; Pain Perception; Working Group; Neural Crest; Behavior; Fish; Mutations; Outbreak
ISSN (print) / ISBN 1545-8547
e-ISSN 1557-8542
Journal Zebrafish
Quellenangaben Volume: 18, Issue: 4, Pages: 282-292 Article Number: , Supplement: ,
Publisher Mary Ann Liebert
Publishing Place 140 Huguenot Street, 3rd Fl, New Rochelle, Ny 10801 Usa
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) CF Comparative Medicine (CF-Comparative Medicine)