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A practical guide to photoswitching optoacoustics tomography.
Methods Enzymol. 657, 365-383 (2021)
Photochromic proteins and photoswitching optoacoustics (OA) are a promising combination, that allows OA imaging of even small numbers of cells in whole live animals and thus can facilitate a more wide-spread use of OA in life-science and preclinical research. The concept relies on exploiting the modulation achieved by the photoswitching to discriminate the agents' signal from the non-modulating background. Here we share our analysis approaches that can be readily used on data generated with commercial OA tomography imaging instrumentation allowing—depending on the used photoswitching agent and sample—routine visualizations of as little as several hundreds of transgene labeled cells per imaging volume in the live animal.
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Publication type
Article: Journal article
Document type
Review
Keywords
Bacteriophytochrome ; Image Analysis ; Imaging ; Optoacoustic ; Photoacoustic ; Photochromic Protein ; Photoswitching ; Transgene Reporter ; Unmixing
ISSN (print) / ISBN
0076-6879
e-ISSN
0076-6879
Journal
Methods in Enzymology
Quellenangaben
Volume: 657,
Pages: 365-383
Publisher
Elsevier
Publishing Place
525 B Street, Suite 1900, San Diego, Ca 92101-4495 Usa
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Biological and Medical Imaging (IBMI)
Grants
DFG