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Flow cytometry detection and quantification of CAR T cells into solid tumors.
Methods Cell Biol. 167, 99-122 (2022)
Adoptive T cell therapy (ACT) is a therapeutic approach which employs genetically manipulated autologous T cells to target and eliminate a patient's malignancy. This novel therapeutic approach, when employing a chimeric antigen receptor (CAR) targeting CD19-expressing B cells, has shown remarkable success in treating acute B-cell lymphocytic leukemia. However, blood born malignancies represent only a fraction of cancers which affect patients. Unfortunately, the utilization of ACT to target solid malignancies has only shown marginal success rates. There are many known obstacles which hinder CAR T cell therapy in patients suffering from solid cancer, one notable obstacle is the effective trafficking of CAR T cells to the tumor site. With the rapid advancement of novel approaches and targets which may enhance CAR T cell infiltration into solid tumors, a standardized approach to assess and measure CAR T cell infiltration becomes imperative in order to compare these different approaches across platforms. Here we describe a flow cytometry method which enables the rapid detection and quantification of CAR T cells which have reached and entered the tumor mass following intravenous injection. Competence with single cell preparation and flow cytometry is required for optimal results.
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Publication type
Article: Journal article
Document type
Review
Keywords
Adoptive T Cell Transfer ; Cancer Immunotherapy ; Car T Cells ; Flow Cytometry ; Immunotherapy ; Protocol ; T Cell Tracking
ISSN (print) / ISBN
0091-679X
Journal
Methods in Cell Biology
Quellenangaben
Volume: 167,
Pages: 99-122
Publisher
Elsevier
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Unit for Clinical Pharmacology (KKG-EKLiP)