PuSH - Publication Server of Helmholtz Zentrum München: SPF45/RBM17-dependent, but not U2AF-dependent, splicing in a distinct subset of human short introns.

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Fukumura, K.* ; Yoshimoto, R.* ; Sperotto, L. ; Kang, H.-S. ; Hirose, T.* ; Inoue, K.* ; Sattler, M. ; Mayeda, A.*

SPF45/RBM17-dependent, but not U2AF-dependent, splicing in a distinct subset of human short introns.

Nat. Commun. 12:4910 (2021)

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Human pre-mRNA introns vary in size from under fifty to over a million nucleotides. We searched for essential factors involved in the splicing of human short introns by screening siRNAs against 154 human nuclear proteins. The splicing activity was assayed with a model HNRNPH1 pre-mRNA containing short 56-nucleotide intron. We identify a known alternative splicing regulator SPF45 (RBM17) as a constitutive splicing factor that is required to splice out this 56-nt intron. Whole-transcriptome sequencing of SPF45-deficient cells reveals that SPF45 is essential in the efficient splicing of many short introns. To initiate the spliceosome assembly on a short intron with the truncated poly-pyrimidine tract, the U2AF-homology motif (UHM) of SPF45 competes out that of U2AF65 (U2AF2) for binding to the UHM-ligand motif (ULM) of the U2 snRNP protein SF3b155 (SF3B1). We propose that splicing in a distinct subset of human short introns depends on SPF45 but not U2AF heterodimer.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords U2af Homology Motif; Proteomic Analysis; Smn Complex; Sex-lethal; Rna; Recognition; Spliceosome; Protein; Mechanisms; Cells

ISSN (print) / ISBN 2041-1723

e-ISSN 2041-1723

Journal Nature Communications

Quellenangaben Volume: 12, Issue: 1, Article Number: 4910

Publisher Nature Publishing Group

Publishing Place London

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Structural Biology (STB)

Grants Kobe University
DFG
JSPS
Nitto Foundation
Hori Sciences and Arts Foundation
Japan Society for the Promotion of Science (JSPS)