PuSH - Publication Server of Helmholtz Zentrum München: <em>Mycobacterium avium</em> subsp. <em>paratuberculosis</em> proteome changes profoundly in milk.

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Kleinwort, K.J.H.* ; Hobmaier, B.F.* ; Mayer, R.* ; Hölzel, C.* ; Degroote, R.L.* ; Märtlbauer, E.* ; Hauck, S.M. ; Deeg, C.A.*

Mycobacterium avium subsp. paratuberculosis proteome changes profoundly in milk.

Metabolites 11:549 (2021)

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Mycobacterium avium subspecies paratuberculosis (MAP) are detectable viable in milk and other dairy products. The molecular mechanisms allowing the adaptation of MAP in these products are still poorly understood. To obtain information about respective adaptation of MAP in milk, we differentially analyzed the proteomes of MAP cultivated for 48 h in either milk at 37 °C or 4 °C or Middlebrook 7H9 broth as a control. From a total of 2197 MAP proteins identified, 242 proteins were at least fivefold higher in abundance in milk. MAP responded to the nutritional shortage in milk with upregulation of 32% of proteins with function in metabolism and 17% in fatty acid metabolism/synthesis. Additionally, MAP upregulated clusters of 19% proteins with roles in stress responses and immune evasion, 19% in transcription/translation, and 13% in bacterial cell wall synthesis. Dut, MmpL4_1, and RecA were only detected in MAP incubated in milk, pointing to very important roles of these proteins for MAP coping with a stressful environment. Dut is essential and plays an exclusive role for growth, MmpL4_1 for virulence through secretion of specific lipids, and RecA for SOS response of mycobacteria. Further, 35 candidates with stable expression in all conditions were detected, which could serve as targets for detection. Data are available via ProteomeXchange with identifier PXD027444.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Lc–ms/ms ; Mycobacterium Avium Subspecies Paratuberculosis ; Dairy Product ; Differential Protein Expression ; Food Safety ; Milk ; Proteomics; Aldehyde Dehydrogenase 2; Hypothetical Proteins; Vibrio-vulnificus; Escherichia-coli; Crohns-disease; Alda-1; Injury; Inactivation; Infection; Survival

ISSN (print) / ISBN 2218-1989

e-ISSN 2218-1989

Journal Metabolites

Quellenangaben Volume: 11, Issue: 8, Article Number: 549

Publisher MDPI

Publishing Place St Alban-anlage 66, Ch-4052 Basel, Switzerland

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) CF Metabolomics & Proteomics (CF-MPC)

Grants Bundesministerium für Wirtschaft und Technologie
Forschungskreis der Ernährungsindustrie